首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠Ⅲ型胶原(Col Ⅲ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的Col Ⅲ含量。Col Ⅲ即Ⅲ型胶原蛋白，它是细胞外基质重要成分，在维持组织器官结构和功能上起关键作用。研究发现其异常表达与多种疾病相关，如纤维化疾病等。机制上，可能通过影响细胞黏附、迁移等行为，参与疾病发生发展过程。试剂盒检测范围为3.12 pg/mL-200 pg/mL，能够满足纤维化疾病机制研究、药物干预效果评估等科研需求。可广泛应用于心血管疾病模型、器官纤维化模型等研究中 Col Ⅲ 动态变化的监测，为探索纤维化相关分子机制提供数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Collagen Type 3,Col 3,_x000D_ Collagen Type III,Col III
缩写：Col Ⅲ
种属：Mus musculus (Mouse)
样本类型：serum, plasma
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Col Ⅲ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:20 | Average % | 89 | ||||
| Range % | 82-96 | |||||
| 1:40 | Average % | 98 | ||||
| Range % | 94-102 | |||||
| 1:80 | Average % | 97 | ||||
| Range % | 90-104 | |||||
| 1:160 | Average % | 86 | ||||
| Range % | 82-90 | |||||
回收率：
| The recovery of mouse Col Ⅲ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 87-99 | ||||
| EDTA plasma (n=4) | 100 | 96-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 2002.5532.5462.5502.357 1001.8751.9541.9151.722 501.2971.3061.3021.109 250.8320.8750.8540.661 12.50.5130.5560.5350.342 6.250.3750.3950.3850.192 3.120.2460.2530.2500.057 00.1880.1970.193 | ||||||
货期：3-5 working days
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