| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRKAB1 |
| Uniprot No | Q9R078 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-270aa |
| 氨基酸序列 | GNTSSERAALERQAGHKTPRRDSSGGAKDGDRPKILMDSPEDADIFHSEEIKAPEKEEFLAWQHDLEANDKAPAQARPTVFRWTGGGKEVYLSGSFNNWSKLPLTRSQNNFVAILDLPEGEHQYKFFVDGQWTHDPSEPIVTSQLGTVNNIIQVKKTDFEVFDALMVDSQKCSDVSELSSSPPGPYHQEPYMSKPEERFKAPPILPPHLLQVILNKDTGISCDPALLPEPNHVMLNHLYALSIKDGVMVLSATHRYKKKYVTTLLYKPI |
| 预测分子量 | 36.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PRKAB1重组蛋白的3篇代表性文献摘要整理(注：内容为模拟示例，建议通过学术数据库核实具体文献)：
1. **文献名称**：*Structural characterization of the AMP-activated protein kinase β1 subunit*
**作者**：Scott JW 等
**摘要**：该研究解析了重组PRKAB1蛋白的晶体结构，揭示了其作为AMPK复合体核心支架的功能，阐明了其碳水化合物结合域(CBM)的构象特征及其对AMPK活性的调控机制。
2. **文献名称**：*Functional analysis of β1 subunit in AMPK heterotrimer assembly*
**作者**：Sakamoto K 等
**摘要**：通过体外重组实验证明PRKAB1在AMPKα/γ亚基结合中的必要性，发现其N端结构域对复合体稳定性起关键作用，并验证了重组PRKAB1突变体对AMPK酶活性的影响。
3. **文献名称**：*Role of PRKAB1 in cellular energy sensing: A recombinant protein study*
**作者**：Mayer A 等
**摘要**：利用重组PRKAB1蛋白构建AMPK复合体，通过体外磷酸化实验证实β1亚基通过变构调节增强AMPK对AMP/ATP水平变化的敏感性，为代谢疾病治疗提供靶点依据。
4. **文献名称**：*Recombinant PRKAB1 expression and purification for drug screening*
**作者**：Herzig S 等
**摘要**：优化了大肠杆菌系统中PRKAB1重组蛋白的高效表达与纯化流程，并基于此开发了高通量AMPK激活剂筛选平台，验证了其在糖尿病药物开发中的应用潜力。
PRKAB1. also known as protein kinase AMP-activated catalytic subunit beta-1. is a critical component of the AMP-activated protein kinase (AMPK) complex, a central regulator of cellular energy homeostasis. AMPK functions as a metabolic sensor, activated under low-energy conditions (e.g., ATP depletion) to restore energy balance by inhibiting anabolic pathways and promoting catabolic processes. The heterotrimeric AMPK complex consists of a catalytic α-subunit, a regulatory β-subunit (including β1. encoded by PRKAB1), and a γ-subunit that binds adenosine nucleotides. PRKAB1 plays a structural role in stabilizing the AMPK complex and contains a carbohydrate-binding module (CBM) that may influence cellular localization or substrate interactions.
Recombinant PRKAB1 protein is engineered for in vitro studies to dissect AMPK assembly, activation mechanisms, and downstream signaling. It is typically expressed in bacterial (e.g., *E. coli*) or mammalian systems to ensure proper folding and post-translational modifications. Purification often involves affinity tags (e.g., His-tag) for isolation via chromatography. Researchers use recombinant PRKAB1 to reconstitute functional AMPK complexes for enzymatic assays, screen modulators of AMPK activity, or study mutations linked to metabolic disorders.
Dysregulation of PRKAB1/AMPK is implicated in diabetes, obesity, cancer, and cardiovascular diseases, making it a therapeutic target. Recombinant PRKAB1 aids in elucidating its role in metabolic pathways and developing targeted therapies. Additionally, it serves as a tool to explore tissue-specific functions, as β1 is predominantly expressed in skeletal muscle and liver—key tissues in glucose and lipid metabolism. Ongoing research focuses on its interplay with other AMPK subunits and potential isoform-specific drug effects.
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