| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PRKAB2 |
| Uniprot No | O43741 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-272aa |
| 氨基酸序列 | MGNTTSDRVSGERHGAKAARSEGAGGHAPGKEHKIMVGSTDDPSVFSLPDSKLPGDKEFVSWQQDLEDSVKPTQQARPTVIRWSEGGKEVFISGSFNNWSTKIPLIKSHNDFVAILDLPEGEHQYKFFVDGQWVHDPSEPVVTSQLGTINNLIHVKKSDFEVFDALKLDSMESSETSCRDLSSSPPGPYGQEMYAFRSEERFKSPPILPPHLLQVILNKDTNISCDPALLPEPNHVMLNHLYALSIKDSVMVLSATHRYKKKYVTTLLYKPI |
| 预测分子量 | 57.3kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PRKAB2重组蛋白的3篇参考文献的简要信息，基于已有知识库整理：
1. **文献名称**: "Expression and Functional Characterization of Recombinant Human AMPK β2 Subunit in Energy Metabolism Regulation"
**作者**: Chen L, et al.
**摘要**: 该研究成功在大肠杆菌中表达并纯化了重组人源PRKAB2蛋白，验证其与AMPK α/γ亚基形成复合物的能力，并证明其在调节细胞葡萄糖摄取和脂肪酸氧化中的关键作用。
2. **文献名称**: "Structural Insights into the AMPK β2 Subunit Oligomerization via Recombinant Protein Analysis"
**作者**: Sanders MJ, et al.
**摘要**: 通过X射线晶体学解析PRKAB2重组蛋白的结构，揭示了β2亚基在AMPK复合物中的二聚化机制及其对激酶活性的调控，为靶向AMPK的药物设计提供理论依据。
3. **文献名称**: "Development of a Mammalian Cell System for High-Yield Production of Functional PRKAB2 Recombinant Protein"
**作者**: Kim S, et al.
**摘要**: 本研究构建了基于HEK293细胞的重组PRKAB2表达体系，优化了纯化流程，获得高活性蛋白，并用于体外筛选AMPK激活剂，证实其在代谢疾病药物开发中的应用潜力。
(注：上述文献为模拟示例，实际引用时请核实真实数据库如PubMed的具体论文。)
PRKAB2 recombinant protein is derived from the PRKAB2 gene, which encodes the beta-2 regulatory subunit of AMP-activated protein kinase (AMPK), a critical energy sensor in eukaryotic cells. AMPK is a heterotrimeric enzyme composed of α (catalytic), β (scaffolding), and γ (nucleotide-binding) subunits, with β-subunits (β1 and β2) playing structural and regulatory roles. The β2 isoform, encoded by PRKAB2. is predominantly expressed in skeletal muscle, heart, and liver, where it stabilizes the AMPK complex and modulates its activity in response to cellular energy fluctuations. Unlike β1. β2 contains a carbohydrate-binding module (CBM) that may influence substrate specificity or localization.
Recombinant PRKAB2 protein is typically produced using expression systems like *E. coli* or mammalian cells, engineered to include affinity tags (e.g., His-tag) for purification. This protein serves as a tool to study AMPK's role in metabolic pathways, including glucose uptake, lipid metabolism, and mitochondrial biogenesis. Researchers use it to investigate how β2-specific interactions affect AMPK activation under stress conditions (e.g., hypoxia, exercise) or in diseases like type 2 diabetes, obesity, and cardiovascular disorders. Its recombinant form also aids in structural studies, drug screening, and developing targeted therapies aiming to modulate AMPK activity. Dysregulation of PRKAB2 has been linked to insulin resistance and cancer progression, highlighting its therapeutic potential. By enabling precise biochemical and functional analyses, PRKAB2 recombinant protein advances our understanding of metabolic regulation and supports the discovery of AMPK-related treatments.
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