| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/50 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/500-1/1000 | Human,Mouse,Rat |
| Aliases | BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del; PTENbeta |
| WB Predicted band size | 47 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human PTEN |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与PTEN抗体相关的经典文献示例(内容为虚构简化，仅供参考)：
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1. **文献名称**: "Comprehensive characterization of PTEN monoclonal antibodies for specificity and sensitivity in clinical diagnostics"
**作者**: Li J. et al.
**摘要**: 比较了多种市售PTEN抗体的特异性，通过Western blot和免疫组化验证其在癌症组织中的检测可靠性，发现部分抗体存在非特异性结合问题，推荐了两种高特异性抗体用于临床样本分析。
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2. **文献名称**: "PTEN expression loss in glioblastoma: Validation of a novel antibody for immunohistochemical analysis"
**作者**: Wang H. et al.
**摘要**: 开发了一种新型PTEN抗体，优化了染色条件，证实其在胶质母细胞瘤中检测PTEN蛋白缺失的敏感性，并与基因测序结果高度一致，为肿瘤分子分型提供可靠工具。
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3. **文献名称**: "Antibody cross-reactivity in PTEN knockout models: Implications for precision research"
**作者**: Martinez R. et al.
**摘要**: 揭示了部分PTEN抗体在小鼠模型中的交叉反应性，强调抗体验证在基因敲除研究中的必要性，提出标准化实验流程以避免假阳性结果。
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4. **文献名称**: "PTEN antibody-based biosensors for real-time monitoring of cellular signaling"
**作者**: Chen L. et al.
**摘要**: 利用高亲和力PTEN抗体开发了新型生物传感器，成功实时追踪PTEN蛋白动态变化，揭示了其在PI3K/AKT通路调控中的快速响应机制。
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(注：以上为模拟文献，实际引用请通过PubMed或学术数据库检索真实研究。)
PTEN (phosphatase and tensin homolog) is a critical tumor suppressor gene that encodes a dual-specificity phosphatase protein involved in regulating cell growth, proliferation, and survival. The PTEN protein primarily antagonizes the PI3K/AKT/mTOR signaling pathway by dephosphorylating phosphatidylinositol (3.4.5)-trisphosphate (PIP3), thereby maintaining cellular homeostasis. Loss or mutation of PTEN is linked to various cancers, metabolic disorders, and neurological conditions, making it a key focus in biomedical research.
PTEN antibodies are essential tools for detecting and quantifying PTEN expression in experimental and clinical settings. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry to study PTEN localization, expression levels, and post-translational modifications. Specific PTEN antibodies target distinct epitopes, such as the N-terminal phosphatidylinositol phosphate-binding domain or the C-terminal regulatory region, allowing researchers to explore structural and functional aspects of the protein.
Validating PTEN antibodies is crucial due to potential cross-reactivity with pseudogenes or homologous proteins. High-quality antibodies are characterized by specificity, sensitivity, and reproducibility across sample types, including formalin-fixed tissues or cell lysates. Clinically, PTEN antibody-based assays aid in diagnosing PTEN-related syndromes (e.g., PTEN hamartoma tumor syndrome) and assessing cancer prognosis. Ongoing research continues to refine PTEN antibody applications, supporting advances in targeted therapies and personalized medicine.
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