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Rat Granulocyte-Macrophage Colony Stimulating Factor,GM-CSF ELISA Kit

  • 中文名： 大鼠粒细胞巨噬细胞集落刺激因子(GM-CSF)ELISA Kit
货号: IPD-E04570r
Price： ￥2500
数量：
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产品详情

  • 产品描述：
    大鼠粒细胞巨噬细胞集落刺激因子(GM-CSF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CSF2含量。CSF2，即粒细胞-巨噬细胞集落刺激因子（GM-CSF），是一种由多种细胞分泌的细胞因子，最初被定义为一种造血因子。它通过调节骨髓细胞生成和免疫反应，在免疫调节、炎症反应和肿瘤进展中扮演关键角色。CSF2通过激活信号通路，如JAK-STAT和PI3K-Akt，影响细胞的增殖、分化和功能，并在多种疾病的治疗中具有潜在应用价值。试剂盒检测范围为15/6 pg/ml- 1000 pg/ml，灵敏度为3.9 pg/ml。适用于科研领域中对大鼠感染性疾病模型、免疫调控机制、造血功能研究以及药物干预效果的评价。实验操作兼容多种生物样本类型，可满足体外实验中GM - CSF的动态监测需求，为探索免疫相关疾病的分子机制或评估实验处理对细胞因子表达的影响提供可靠工具，广泛应用于基础医学、药理学及免疫学等研究场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    Csf2 ELISA kit; CsfgmGranulocyte-macrophage colony-stimulating factor ELISA kit; GM-CSF ELISA kit; Colony-stimulating factor ELISA kit; CSF ELISA kit
  • 缩写：
    CSF2
  • Uniprot No.：
    P48750
  • 种属：
    Rattus norvegicus (Rat)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    15.6 pg/ml - 1000 pg/ml
  • 灵敏度：
    3.9 pg/ml
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度：

    To assess the linearity of the assay, samples were spiked with high concentrations of rat GM-CSF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    85


    Range %

    81-89


    1:2

    Average %

    97


    Range %

    92-101


    1:4

    Average %

    91


    Range %

    86-95


    1:8

    Average %

    103


    Range %

    99-107


  • 回收率：

    The recovery of rat GM-CSF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    88

    84-92


    EDTA plasma (n=4)

    94

    90-98


  • 标准曲线：

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    pg/ml

    OD1

    OD2

    Average

    Corrected


    1000

    2.468

    2.326

    2.397

    2.210


    500

    1.857

    1.792

    1.825

    1.638


    250

    1.213

    1.250

    1.232

    1.045


    125

    0.785

    0.813

    0.799

    0.612


    62.5

    0.492

    0.468

    0.480

    0.293


    31.2

    0.331

    0.347

    0.339

    0.152


    15.6

    0.289

    0.271

    0.280

    0.093


    0

    0.184

    0.189

    0.187




  • 货期：
    3-5 working days

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