首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠结缔组织生长因子(CTGF)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的CTGF含量。CTGF即结缔组织生长因子，在细胞增殖、分化、黏附及血管生成等多种生理病理过程中发挥作用。其过表达与多种纤维化疾病及肿瘤发展密切相关。研究机制聚焦于其信号通路，如TGF -β/Smad途径等，以探寻干预靶点治疗相关疾病。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于体外研究大鼠模型中的CTGF动态表达分析，例如纤维化疾病机制探索、药物干预效果评估或细胞因子网络研究，可为心血管、肝肾疾病等科研领域提供可靠的实验工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ccn2 ELISA Kit; CtgfCCN family member 2 ELISA Kit; Cellular communication network factor 2 ELISA Kit; Connective tissue growth factor ELISA Kit; Connective tissue growth-related protein ELISA Kit
缩写：CTGF
Uniprot No.：Q9R1E9
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat CTGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 85-96 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 91-98 | |||||
| 1:4 | Average % | 85 | ||||
| Range % | 89-100 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-99 | |||||
回收率：
| The recovery of rat CTGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-96 | ||||
| EDTA plasma (n=4) | 96 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.0971.9872.0421.88051.5681.4881.5281.3662.51.0071.0011.0040.8421.250.6370.6270.6320.4700.6250.3700.3600.3650.2030.3120.3010.2920.2970.1350.1560.2440.2390.2420.08000.1620.1610.162 | ||||||
货期：3-5 working days
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