| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | Nek8; NERCC; NERCC1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human NEK9 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于NEK9抗体的参考文献，包含文献名称、作者及摘要概括：
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1. **文献名称**：*NEK9 regulates primary cilia formation by acting as a selective autophagy adaptor for MYH9/myosin IIA*
**作者**：R. Roig et al.
**摘要**：该研究利用NEK9特异性抗体，通过免疫荧光和免疫沉淀技术，揭示了NEK9通过调控自噬途径降解MYH9蛋白，影响纤毛形成和细胞周期进程的分子机制。
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2. **文献名称**：*A functional interplay between Aurora A kinase and Nek9 in the control of mitotic cell division*
**作者**：A. Bertran et al.
**摘要**：研究通过Western blot和共聚焦显微镜结合NEK9抗体，发现NEK9与Aurora A激酶相互作用，共同调控有丝分裂纺锤体组装及染色体分离的准确性。
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3. **文献名称**：*Nek9 is a multifunctional regulator of actomyosin dynamics during epithelial morphogenesis*
**作者**：M. Graser et al.
**摘要**：利用NEK9抗体进行蛋白质组学分析，证明NEK9通过磷酸化肌球蛋白轻链(MLC)调控上皮细胞形态发生，影响胚胎发育中的细胞迁移和极性建立。
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这些文献均通过NEK9抗体探究其在细胞分裂、自噬及发育中的功能，涵盖分子机制与疾病关联研究。如需具体DOI或年份信息可进一步补充。
**Background of NEK9 Antibody**
NEK9 (NIMA-related kinase 9) is a serine/threonine kinase belonging to the NEK family, which plays critical roles in cell cycle regulation, particularly during mitosis. It is involved in key processes such as centrosome maturation, mitotic spindle assembly, and chromosome segregation. NEK9 functions upstream of NEK6 and NEK7. activating these kinases through phosphorylation, which are essential for mitotic progression. Dysregulation of NEK9 has been linked to genomic instability and is implicated in cancers, including breast, ovarian, and neuroblastoma, where its overexpression correlates with poor prognosis.
NEK9 antibodies are essential tools for studying its expression, localization, and function in cellular and disease contexts. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate NEK9's role in cell cycle checkpoints, its interaction with binding partners (e.g., BRCA1. HSP90), and its phosphorylation dynamics. Research utilizing NEK9 antibodies has also explored its potential as a therapeutic target, particularly in cancer, where kinase inhibitors or siRNA-mediated knockdown are being tested. These studies highlight NEK9's dual role as a regulator of mitotic fidelity and a contributor to oncogenic pathways, making it a focus of both basic and translational research.
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