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产品描述：人维生素D25羟化酶(CYP2R1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的CYP2R1含量。CYP2R1是一种关键靶点。它参与维生素D的代谢过程，在调节钙磷平衡、维持骨骼健康等方面起重要作用。研究其机制主要聚焦于它在维生素D羟化步骤中的功能，明确其作用有助于深入了解相关疾病发病机制并开发新疗法。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于科研领域中对维生素D代谢机制、营养缺乏性疾病模型、骨骼发育异常或免疫相关疾病的机制研究，亦可用于药物开发过程中对CYP2R1表达水平的动态监测本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CP2R1_HUMAN ELISA Kit; CYP2R1 ELISA Kit; Cytochrome P450 2R1 ELISA Kit; Cytochrome P450 family 2 R1 ELISA Kit; Cytochrome P450 family 2 subfamily R polypeptide 1 ELISA Kit; EC 1.14.13.15 ELISA Kit; MGC4663 ELISA Kit; Vitamin D 25 hydroxylase ELISA Kit; Vitamin D 25-hydroxylase ELISA Kit
缩写：CYP2R1
Uniprot No.：Q6VVX0
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of Human CYP2R1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 102 | ||||
| Range % | 98-106 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 89-98 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 89-96 | |||||
| 1:8 | Average % | 87 | ||||
| Range % | 81-93 | |||||
回收率：
| The recovery of Human CYP2R1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-100 | ||||
| EDTA plasma (n=4) | 87 | 83-91 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.5082.7142.6112.4765001.8931.7851.8391.7042501.1471.1831.1651.0301250.6470.6650.6560.52162.50.3740.3820.3780.24331.20.2970.2890.2930.15815.60.2210.2250.2230.08800.1340.1350.135 | ||||||
货期：3-5 working days
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