首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人细胞色素P450 3A4(CYP3A4)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CYP3A4含量。CYP3A4 是细胞色素 P450 酶家族成员，在人体药物代谢中起关键作用。它能氧化多种内源性和外源性物质。研究机制聚焦于其底物特异性、代谢途径、与药物相互作用等方面，了解这些有助于优化药物疗效、降低不良反应。试剂盒检测范围为23.5 pg/mL-1500 pg/mL，适用于药物代谢机制研究、体外肝细胞模型评估、药代动力学分析及环境毒物对代谢酶调控作用等科研领域，为研究CYP3A4介导的代谢通路提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CYP3A4; CYP3A3; Cytochrome P450 3A4; 1,4-cineole 2-exo-monooxygenase; 1,8-cineole 2-exo-monooxygenase; Albendazole monooxygenase; sulfoxide-forming; Albendazole sulfoxidase; CYPIIIA3; CYPIIIA4; Cholesterol 25-hydroxylase; Cytochrome P450 3A3; Cytochrome P450 HLp; Cytochrome P450 NF-25; Cytochrome P450-PCN1; Nifedipine oxidase; Quinine 3-monooxygenase
缩写：CYP3A4
Uniprot No.：P08684
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：23.5 pg/mL-1500 pg/mL
灵敏度：5.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CYP3A4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 85-99 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 90-102 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 89-105 | |||||
| 1:5 | Average % | 95 | ||||
| Range % | 90-100 | |||||
回收率：
| The recovery of human CYP3A4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 94-103 | ||||
| EDTA plasma (n=4) | 98 | 95-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected15002.7542.7912.7732.5977502.3692.3942.3822.2063751.8241.7891.8071.631187.51.0571.0821.0700.894940.6400.6470.6440.468470.4350.4650.4500.27423.50.2640.2780.2710.09500.1750.1760.176 | ||||||
货期：3-5 working days
×
