首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人细胞色素P450 (CYP450)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CYP450含量。CYP450是一种广泛存在于肝微粒体的药物代谢酶，参与脂肪酸、类花生四烯酸等多种内源性物质及药物、毒物、致癌物等外源性化合物的代谢。其研究机制主要涉及基因多态性、酶活性与药物代谢的关系，对药物疗效和毒性有重要影响。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，本试剂盒适用于体外实验，可用于探索药物诱导或抑制CYP450活性机制、评估化合物代谢稳定性、研究疾病模型中代谢酶表达变化等科研场景，为细胞代谢调控、药物开发基础研究及环境毒理分析提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AHH ELISA Kit; AHRR ELISA Kit; Aryl hydrocarbon hydroxylase ELISA Kit; CP11 ELISA Kit; CP1A1_HUMAN ELISA Kit; CYP 1 ELISA Kit; CYP1 ELISA Kit; cyp1a1 ELISA Kit; CYPIA1 ELISA Kit; Cytochrome P1 450 dioxin inducible ELISA Kit; Cytochrome P1-450 ELISA Kit; Cytochrome P450 1A1 ELISA Kit; Cytochrome P450 family 1 subfamily A polypeptide 1 ELISA Kit; Cytochrome P450 form 6 ELISA Kit; Cytochrome P450 subfamily I (aromatic compound inducible) polypeptide 1 ELISA Kit; Cytochrome P450-C ELISA Kit; Cytochrome P450-P1 ELISA Kit; Flavoprotein-linked monooxygenase ELISA Kit; Microsomal monooxygenase ELISA Kit; P1 450 ELISA Kit; P450 C ELISA Kit; P450 form 6 ELISA Kit; P450 P1 ELISA Kit; P450DX ELISA Kit; Xenobiotic monooxygenase ELISA Kit
缩写：CYP450
Uniprot No.：P04798
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human Cytochrome P450 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 93 | |||||
| Range % | 88-97 | ||||||
| 1:2 | Average % | 104 | |||||
| Range % | 100-112 | ||||||
| 1:4 | Average % | 95 | |||||
| Range % | 90-100 | ||||||
| 1:8 | Average % | 84 | |||||
| Range % | 80-89 | ||||||
回收率：
| The recovery of human Cytochrome P450 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 98 | 94-102 | |||||
| EDTA plasma (n=4) | 89 | 84-93 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected20002.4072.5322.4702.31910001.6111.6841.6481.4975001.0521.0051.0290.8782500.6090.5930.6010.4501250.3950.4190.4070.25662.50.3130.3260.3200.16931.250.2680.2510.2600.10900.1540.1470.151 | |||||||
货期：3-5 working days
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