首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人胆固醇7α-羟化酶（CYP7A）酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的CYP7A1含量。CYP7A1是胆固醇代谢关键酶，位于肝脏，其表达受基因多态性、饮食、激素等因素调控。研究显示，CYP7A1通过调节胆固醇转化为胆汁酸，影响血脂代谢，与多种疾病及治疗反应相关。试剂盒检测范围为18.75 pg/mL-1200 pg/mL，该产品适用于基础医学研究领域，包括但不限于肝胆系统疾病机制探索、脂质代谢调控网络解析、药物或膳食成分对胆汁酸合成途径的影响评估等科研场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cholesterol 7 alpha hydroxylase ELISA Kit; Cholesterol 7 alpha monooxygenase ELISA Kit; Cholesterol 7-alpha-hydroxylase ELISA Kit; Cholesterol 7-alpha-monooxygenase ELISA Kit; CP7A ELISA Kit; CP7A1_HUMAN ELISA Kit; CYP 7 ELISA Kit; CYP7 ELISA Kit; CYP7A1 ELISA Kit; CYPVII ELISA Kit; Cytochrome P450 7A1 ELISA Kit; Cytochrome P450; family 7; subfamily A; polypeptide 1 ELISA Kit; Cytochrome P450; subfamily VIIA (cholesterol 7 alpha monooxygenase); ELISA Kit; Cytochrome P450; subfamily VIIA (cholesterol 7 alpha-monooxygenase); polypeptide 1 ELISA Kit; MGC126826 ELISA Kit; MGC138389 ELISA Kit
缩写：CYP7A1
Uniprot No.：P22680
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：18.75 pg/mL-1200 pg/mL
灵敏度：4.7 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human CYP7A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 80-90 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 90-100 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 85-97 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 87-98 | |||||
回收率：
| The recovery of human CYP7A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 87-98 | ||||
| EDTA plasma (n=4) | 91 | 85-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected12002.6062.5202.5632.4206001.9121.7691.8411.6983001.0701.0041.0370.8941500.6860.6390.6630.520750.4080.3940.4010.25837.50.3140.3090.3120.16918.750.2160.1990.2080.06500.1490.1370.143 | ||||||
货期：3-5 working days
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