首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人β防御素104(DEFB104A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的DEFB104A含量。DEFB104A是一个靶点。其它可能在人体免疫等生理过程中有潜在作用。研究机制上，可能涉及参与免疫细胞的调控、抗菌防御等反应，但目前关于它的详细背景及具体研究机制还需更多研究来进一步明确和完善。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，尤其适用于感染性疾病模型、免疫调控机制研究以及抗菌肽相关的基础科研，能够为体外实验模型中DEFB104A的动态变化提供可靠的定量数据支持，助力宿主防御机制及炎症相关通路的探索。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：BD 4 ELISA Kit; BD-4 ELISA Kit; BD4 ELISA Kit; beta 104 ELISA Kit; beta 4 Defensin ELISA Kit; Beta defensin 4 ELISA Kit; Beta-defensin 104 ELISA Kit; Beta-defensin 4 ELISA Kit; D104A_HUMAN ELISA Kit; DEFB-4 ELISA Kit; DEFB104B ELISA Kit; Defensin ELISA Kit; Defensin beta 104 ELISA Kit; Defensin beta 4 precursor ELISA Kit; hBD 4 ELISA Kit; hBD-4 ELISA Kit; hBD4 ELISA Kit
缩写：DEFB104A
Uniprot No.：Q8WTQ1
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human DEFB104A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-97 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 87-100 | |||||
| 1:4 | Average % | 85 | ||||
| Range % | 81-93 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human DEFB104A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 84-96 | ||||
| EDTA plasma (n=4) | 92 | 85-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.7062.6552.6812.52510002.1362.0112.0741.9185001.3461.2891.3181.1622500.7640.7520.7580.6021250.3670.3540.3610.20562.50.2790.2870.2830.12731.250.2440.2310.2380.08200.1620.1500.156 | ||||||
货期：3-5 working days
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