首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠β-防御素1酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的DEFB1含量。DEFB1（防御素β1）是一种抗菌肽，由中性粒细胞产生，与上皮表面对抗微生物定植的抵抗力有关。其基因表达与多种疾病相关，如龋齿、牙周炎和囊性纤维化。研究显示，DEFB1在男性生育过程中发挥促进精子活力和杀菌的双重作用，其缺陷可能导致弱精子症和精液白细胞增多症。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于科研场景中探究β-防御素1在感染性疾病模型、炎症反应机制或免疫调节研究中的表达变化，例如评估病原体刺激下组织局部防御素分泌动态、分析免疫相关基因敲除动物模型的黏膜屏障功能，或体外研究细胞因子对防御素表达的调控作用本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Defb1Beta-defensin 1 ELISA kit; BD-1 ELISA kit; mBD-1 ELISA kit; Defensin ELISA kit; beta 1 ELISA kit
缩写：DEFB1
Uniprot No.：P56386
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Microbiology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse β-defensins 1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 93 | ||||
| Range % | 89-106 | |||||
| 1:10 | Average % | 92 | ||||
| Range % | 83-100 | |||||
| 1:20 | Average % | 95 | ||||
| Range % | 85-110 | |||||
| 1:40 | Average % | 94 | ||||
| Range % | 89-103 | |||||
回收率：
| The recovery of mouse β-defensins 1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 91-103 | ||||
| EDTA plasma (n=4) | 100 | 95-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 10002.1842.0522.1181.936 5001.3631.3771.3701.188 2500.8900.8610.8760.694 1250.5170.4820.5000.318 62.50.3750.3640.3700.188 31.20.2860.2670.2770.095 15.60.2310.2250.2280.046 00.1840.1790.182 | ||||||
货期：3-5 working days
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