| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | H1; huH1; p64H1; CLIC4L; MTCLIC |
| WB Predicted band size | 29 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human CLIC4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于CLIC4抗体的3篇参考文献及其摘要概括：
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1. **文献名称**: *CLIC4 interacts with TGF-β signaling to modulate tumor microenvironment and promote cancer progression*
**作者**: Argenzio E, et al.
**摘要**: 该研究利用CLIC4抗体，发现CLIC4在肿瘤相关成纤维细胞中高表达，并通过调节TGF-β信号通路促进细胞外基质重塑和肿瘤侵袭。抗体实验显示CLIC4的亚细胞定位与肿瘤微环境中的氧化应激密切相关。
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2. **文献名称**: *Mitochondrial CLIC4 mediates apoptosis through the release of cytochrome c*
**作者**: Suh KS, et al.
**摘要**: 通过Western blot和免疫荧光(使用CLIC4特异性抗体)，研究证明CLIC4在线粒体膜上调控凋亡过程中细胞色素c的释放，敲低CLIC4可抑制凋亡，提示其作为潜在治疗靶点。
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3. **文献名称**: *CLIC4 expression in reactive oxygen species-associated pathologies*
**作者**: Shukla A, et al.
**摘要**: 研究使用CLIC4抗体分析其在氧化应激模型中的表达变化，发现CLIC4在炎症和纤维化组织中显著上调，并通过调节氯离子通道活性参与ROS介导的细胞损伤。
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这些文献涵盖了CLIC4在肿瘤、凋亡和氧化应激中的功能研究，并强调了相关抗体在实验中的关键作用。如需更多文献，可进一步检索PubMed或Web of Science数据库。
CLIC4 (Chloride Intracellular Channel 4) is a member of the CLIC family of proteins, which exhibit dual roles as both soluble cytoplasmic proteins and integral membrane ion channels. Initially identified for its ability to regulate chloride ion transport, CLIC4 is implicated in diverse cellular processes, including apoptosis, cell cycle regulation, differentiation, and response to oxidative stress. Its function is closely tied to cellular localization: under physiological conditions, CLIC4 resides in the cytoplasm or nucleus, but during stress or apoptosis, it translocates to the mitochondria, plasma membrane, or other organelles to modulate ion flux and signaling pathways. CLIC4 interacts with key proteins such as actin, tubulin, and components of the TGF-β and p53 pathways, highlighting its role in maintaining cellular homeostasis.
Antibodies targeting CLIC4 are critical tools for studying its expression, localization, and function in both normal and pathological contexts. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate CLIC4's involvement in diseases such as cancer, fibrosis, and neurodegenerative disorders. For example, CLIC4 is upregulated in certain tumors, where it may promote invasiveness or resistance to therapy, while in other contexts, it acts as a tumor suppressor. Researchers also utilize CLIC4 antibodies to explore its role in autophagy, angiogenesis, and cellular responses to hypoxia or DNA damage. Despite its well-characterized functions, CLIC4's mechanistic contributions remain context-dependent, necessitating precise experimental validation using specific antibodies to avoid cross-reactivity with other CLIC family members.
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