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产品描述：人β-防御素2酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、尿液、组织培养上清液、组织匀浆样本中的DEFB4含量。DEFB4即β-防御素 4，是一种内源性抗菌肽。它在机体固有免疫中起关键作用，具有广谱抗菌活性，能抵御细菌、真菌等病原体。其研究机制主要聚焦在它与病原体膜相互作用、免疫调节功能及信号通路激活等方面，为抗感染治疗提供新思路。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，该产品适用于科研领域对感染性病原体应答机制、慢性炎症性疾病（如银屑病、特应性皮炎）的病理研究，也可用于评估药物或生物制剂对 hBD - 2 分泌的调控作用，为免疫调节、黏膜屏障功能及感染免疫方向的体外实验提供可靠工具。样本兼容性广泛，支持多种生物样本类型的稳定检测，操作流程标准化，适用于实验室常规科研需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：BD 2 ELISA Kit; BD-2 ELISA Kit; BD2 ELISA Kit; beta 2 ELISA Kit; beta 2 Defensin ELISA Kit; Beta defensin 2 ELISA Kit; Beta defensin 4B ELISA Kit; Beta-defensin 2 ELISA Kit; Beta-defensin 4A ELISA Kit; DEF B2 ELISA Kit; DEF B4 ELISA Kit; DEFB 102 ELISA Kit; DEFB 2 ELISA Kit; DEFB 4 ELISA Kit; DEFB102 ELISA Kit; DEFB2 ELISA Kit; DEFB2; formerly ELISA Kit; DEFB4 ELISA Kit; DEFB4; formerly ELISA Kit; DEFB4B ELISA Kit; DEFB4P ELISA Kit; Defensin ELISA Kit; Defensin beta 2 ELISA Kit; Defensin; beta 4 ELISA Kit; Defensin; beta 4; pseudogene ELISA Kit; Defensin; beta 4A ELISA Kit; Defensin; beta 4B ELISA Kit; Defensin; beta; 2; formerly ELISA Kit; Defensin; beta; 4; formerly ELISA Kit; DFB4A_HUMAN ELISA Kit; HBD 2 ELISA Kit; hBD-2 ELISA Kit; HBD2 ELISA Kit; mBD-2 ELISA Kit; SAP 1 ELISA Kit; SAP1 ELISA Kit; Skin antimicrobial peptide 1 ELISA Kit; Skin-antimicrobial peptide 1 ELISA Kit
缩写：DEFB4
Uniprot No.：O15263
种属：Homo sapiens (Human)
样本类型：serum, urine, cell culture supernates, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Microbiology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human β-defensins 2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 81-92 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 90-100 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 91-110 | |||||
| 1:8 | Average % | 91 | ||||
| Range % | 85-98 | |||||
回收率：
| The recovery of human β-defensins 2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected40002.8212.7522.7872.68920002.1822.0762.1292.03110001.4381.3641.4011.3035000.8020.7930.7980.7002500.4710.4370.4540.3561250.3020.2900.2960.19862.50.2430.2290.2360.13800.0990.0970.098 | ||||||
本试剂盒所含材料：
A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human DEFB4 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled DEFB4 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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