Rabbit Polyclonal POLA1 Antibody

POLA1 (DNA Polymerase Alpha 1) is a critical subunit of the DNA polymerase alpha-primase complex, essential for initiating DNA replication in eukaryotic cells. This complex synthesizes short RNA-DNA primers during the synthesis of the leading and lagging strands, providing a 3'-OH terminus for processive DNA polymerases. POLA1. the catalytic subunit, possesses DNA polymerase activity, while its regulatory subunits contribute to RNA primer formation. Antibodies targeting POLA1 are widely used in molecular biology and cancer research to study DNA replication mechanisms, cell cycle regulation, and genome stability. Dysregulation of POLA1 has been linked to replication stress, genomic instability, and diseases such as cancer, where its overexpression is observed in certain malignancies. POLA1 antibodies enable detection of protein expression levels via techniques like Western blotting, immunohistochemistry, and immunofluorescence, aiding in investigations of replication fidelity, DNA damage responses, and therapeutic targeting. Recent studies also explore POLA1's role in antiviral immunity and its potential as a biomarker for cancer prognosis. These antibodies are crucial tools for dissecting POLA1's interactions with replication origin sites, checkpoint proteins, and its involvement in pathologies linked to replication errors.