| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | Q99; EIF2C; EIF2C1; GERP95 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human AGO1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于AGO1抗体的3篇参考文献摘要信息：
1. **文献名称**: "Characterization of a highly specific monoclonal antibody against human Argonaute-1 (AGO1)"
**作者**: Smith J, et al.
**摘要**: 本研究报道了一种针对人AGO1蛋白的高特异性单克隆抗体的开发与验证。通过Western blot和免疫共沉淀实验证明该抗体可特异性识别内源性AGO1.且在多种细胞系中无交叉反应性。应用于miRNA功能研究中成功追踪RISC复合体动态。
2. **文献名称**: "AGO1 antibody validation for RNA-induced silencing complex studies"
**作者**: Lee S, Kim T.
**摘要**: 系统评估了市售AGO1抗体的特异性，通过CRISPR/Cas9敲除细胞系验证抗体信号消失。研究强调了不同抗体在染色质免疫沉淀(ChIP)和免疫荧光中的性能差异，为选择合适试剂提供了依据。
3. **文献名称**: "Dysregulation of AGO1 in hepatocellular carcinoma revealed by immunohistochemical analysis"
**作者**: Chen X, et al.
**摘要**: 采用经验证的AGO1抗体进行肝癌组织芯片分析，发现AGO1蛋白表达水平与患者预后显著相关。研究同时证明该抗体在福尔马林固定石蜡包埋样本中的适用性，为临床病理研究提供工具。
注：以上文献信息为示例性质，实际文献需通过PubMed/Google Scholar检索获取。建议使用关键词"AGO1 antibody validation"+"specificity"或结合研究领域(如癌症、干细胞)进行精准筛选。
AGO1 (Argonaute-1) is a key component of the RNA-induced silencing complex (RISC), playing a central role in post-transcriptional gene regulation through microRNA (miRNA)-mediated silencing. As a member of the Argonaute protein family, AGO1 binds small non-coding RNAs, such as miRNAs or small interfering RNAs (siRNAs), to guide RISC to complementary mRNA targets. This interaction typically leads to translational repression or degradation of the target mRNA, thereby modulating gene expression. AGO1 is widely expressed across tissues and is evolutionarily conserved, underscoring its fundamental role in cellular processes, including development, differentiation, and stress responses.
AGO1-specific antibodies are essential tools for studying miRNA mechanisms, RISC assembly, and gene silencing pathways. Researchers use these antibodies in techniques like immunoprecipitation (e.g., RIP-Seq to identify miRNA targets), Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP). Dysregulation of AGO1 has been linked to diseases such as cancer, neurological disorders, and viral infections, making its detection and quantification critical for both basic research and clinical investigations. Commercially available AGO1 antibodies are typically validated for specificity against paralogs (e.g., AGO2. which has endonucleolytic activity in siRNA pathways) and cross-reactivity across species (human, mouse, rat). However, users must verify experimental conditions, as AGO1's function can overlap with other Argonaute proteins, and antibody performance may vary depending on sample preparation and assay type.
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