| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | DAPAT; DAP-AT; DHAPAT |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human GNPAT |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于GNPAT抗体的3篇参考文献，按文献名称、作者及摘要内容概括整理：
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1. **文献名称**：*Immunochemical characterization of peroxisomal enzymes in rhizomelic chondrodysplasia punctata*
**作者**：Wanders, R.J.A. et al.
**摘要**：该研究通过免疫印迹和酶活性分析，验证了GNPAT抗体在诊断RCDP(一种过氧化物酶体疾病)中的应用，发现患者细胞中GNPAT蛋白水平显著降低，为疾病分型提供了依据。
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2. **文献名称**：*Molecular cloning and functional characterization of human glyceronephosphate O-acyltransferase*
**作者**：de Vet, E.C.J.M. et al.
**摘要**：文章报道了GNPAT基因的克隆与功能研究，通过特异性抗体证实其在过氧化物酶体中的定位，并揭示其催化醚脂合成关键步骤的作用，为后续抗体工具的开发奠定基础。
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3. **文献名称**：*Biomarker discovery for peroxisomal disorders using proteomic approaches*
**作者**：Ferdinandusse, S. et al.
**摘要**：该研究利用GNPAT抗体进行蛋白质组学分析，筛选过氧化物酶体疾病患者的生物标志物，发现GNPAT表达异常与多种代谢紊乱相关，强调了抗体在诊断中的潜在价值。
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**备注**：GNPAT抗体相关研究多集中于过氧化物酶体疾病机制及诊断领域，上述文献均发表于生物化学或遗传学期刊，内容涵盖抗体在基础研究及临床中的应用。若需更多文献，可进一步筛查近年相关主题的蛋白质功能或疾病机制研究。
The GNPAT (glyceronephosphate O-acyltransferase) antibody is a tool used to detect and study the GNPAT protein, a key enzyme in the biosynthesis of plasmalogens—a class of ether phospholipids critical for cell membrane structure, signaling, and organelle function. GNPAT catalyzes the first committed step in plasmalogen synthesis, transferring an acyl group to glyceronephosphate within peroxisomes. Plasmalogens are particularly abundant in tissues with high metabolic activity, such as the brain, heart, and immune cells, and their deficiency is linked to peroxisomal disorders like rhizomelic chondrodysplasia punctata (RCDP) and neurodegenerative diseases.
Research involving GNPAT antibodies focuses on understanding its role in lipid metabolism, peroxisomal function, and disease mechanisms. Mutations in the GNPAT gene are associated with RCDP type 2. a rare genetic disorder characterized by skeletal abnormalities, cataracts, and developmental delays. The antibody is utilized in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess protein expression, localization, and dysregulation in disease models or clinical samples. Additionally, it aids in exploring GNPAT's involvement in oxidative stress responses and its potential crosstalk with metabolic pathways. As plasmalogens are increasingly implicated in neuroprotection and lipid homeostasis, GNPAT antibodies serve as vital reagents for both basic research and diagnostic investigations targeting peroxisome-related pathologies.
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