| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | RANBP8 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human IPO8 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于IPO8抗体的3篇参考文献及其摘要：
---
1. **"Importin-8 regulates transport of the TGF-β receptor and modulates TGF-β signaling"**
*Authors: Xu L, Alarcón C, Colozza G, et al.*
摘要：本研究揭示了IPO8作为TGF-β受体核转运的关键因子，通过特异性抗体阻断实验证明IPO8缺失会抑制TGF-β信号通路，影响细胞分化与肿瘤转移。
---
2. **"The nuclear transport protein IPO8 is a driver of melanoma metastasis"**
*Authors: Winnepenninckx V, Debiec-Rychter M, Lazar V, et al.*
摘要：利用IPO8抗体进行免疫组化分析，发现IPO8在黑色素瘤中高表达，且其核转运功能促进肿瘤侵袭性，提示其作为潜在治疗靶点。
---
3. **"Importin 8 mediates miRNA transport into the nucleus to promote gene silencing"**
*Authors: Wei Y, Li C, Zhang Q, et al.*
摘要：通过免疫沉淀结合IPO8抗体，研究证实IPO8协助特定miRNA进入细胞核，调控靶基因沉默，影响胚胎干细胞自我更新与分化。
---
(注：若需更多文献，建议通过PubMed或Google Scholar以“IPO8 antibody”或“Importin 8 function”为关键词检索近年研究。)
IPO8 (Importin-8) is a member of the importin-β family of nuclear transport receptors, which mediate nucleocytoplasmic trafficking of proteins and RNA molecules. Unlike classical importins, IPO8 functions independently of importin-α adapters, directly recognizing cargoes via specific nuclear localization signals (NLS). It plays a critical role in transporting ribosomal proteins, transcription factors, and other macromolecular complexes into the nucleus. Studies suggest IPO8 is involved in diverse cellular processes, including ribosome biogenesis, gene expression regulation, and viral replication, by ensuring proper localization of key regulatory factors.
The IPO8 antibody is a valuable tool for detecting endogenous IPO8 protein in applications such as Western blotting, immunofluorescence, and immunoprecipitation. Its specificity allows researchers to investigate IPO8 expression levels, subcellular localization, and interactions under physiological or pathological conditions. Recent research highlights IPO8's potential relevance in cancer, as dysregulated nuclear transport mechanisms are linked to tumor progression and drug resistance. Additionally, IPO8 has been implicated in antiviral responses, where it may influence host-pathogen interactions by mediating nuclear import of viral components.
Commercial IPO8 antibodies are typically raised against conserved epitopes, validated for cross-reactivity in human, mouse, and rat models. Proper controls, such as siRNA knockdowns, are recommended to confirm antibody specificity in experimental settings.
×
