| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/10000 | Human,Mouse,Rat |
| Aliases | KIF4; KIF4G1 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human KIF4A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与KIF4A抗体相关的文献摘要概括(文献标题及作者为虚构示例，仅供格式参考)：
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1. **文献名称**: "KIF4A regulates chromosome condensation in mitosis via antibody-based localization analysis"
**作者**: Chen L. et al. (2020)
**摘要**: 通过使用KIF4A特异性抗体进行免疫荧光染色，研究发现KIF4A在中期染色体组装中起关键作用，其缺失导致染色体凝聚异常和细胞分裂失败。
2. **文献名称**: "KIF4A as a biomarker in cervical cancer: Antibody validation and clinical correlation"
**作者**: Gupta S. et al. (2019)
**摘要**: 通过Western blot和免疫组化验证KIF4A抗体的特异性，发现KIF4A在宫颈癌组织中高表达，与患者预后不良相关，提示其作为癌症治疗靶点的潜力。
3. **文献名称**: "KIF4A interacts with Aurora B kinase: Co-immunoprecipitation studies using specific antibodies"
**作者**: Tanaka R. et al. (2018)
**摘要**: 利用KIF4A抗体进行免疫共沉淀实验，证实KIF4A与Aurora B激酶在调控纺锤体组装检查点中的功能互作，揭示了有丝分裂纠错机制的新机制。
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注：以上文献为模拟内容，实际研究中建议通过PubMed或Web of Science以“KIF4A antibody”为关键词检索真实文献。
The KIF4A antibody is a tool used to detect kinesin family member 4A (KIF4A), a microtubule-associated motor protein belonging to the kinesin-4 subfamily. KIF4A plays critical roles in mitotic processes, including spindle assembly, chromosome condensation, and segregation during cell division. It interacts with chromosomal passenger complexes and regulates the spatial organization of chromosomes. Structurally, KIF4A contains an N-terminal motor domain for ATP-dependent microtubule movement and a C-terminal coiled-coil domain mediating protein-protein interactions.
Research highlights KIF4A's dual functionality: it stabilizes chromatin architecture during metaphase and facilitates chromosome "individualization" in anaphase. Dysregulation of KIF4A is linked to genomic instability and carcinogenesis. Overexpression has been observed in various cancers (e.g., breast, lung, gastric), correlating with poor prognosis, tumor progression, and chemoresistance. Conversely, reduced expression impairs cell division, leading to mitotic errors.
KIF4A antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to study its expression patterns, subcellular localization, and interactions with partners like Survivin and Aurora B kinase. These studies contribute to understanding cell cycle regulation, cancer mechanisms, and potential therapeutic targets. Recent investigations also explore its non-mitotic roles in DNA repair and postmitotic neuronal development. Commercial KIF4A antibodies are typically validated for specificity across human, mouse, and rat samples.
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