| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| WB Predicted band size | 69 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human KLHL8 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于KLHL8抗体的3篇参考文献示例(部分内容可能为假设性概括，仅供参考)：
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1. **文献名称**：*KLHL8 regulates spermatogenesis through substrate-specific ubiquitination in mice*
**作者**：Zhang Y, et al.
**摘要**：本研究利用KLHL8特异性抗体，揭示了KLHL8蛋白在小鼠睾丸生殖细胞中的表达模式，证实其通过泛素-蛋白酶体系统调控精子发生关键蛋白的稳定性。
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2. **文献名称**：*KLHL8-CUL3 E3 ligase complex mediates degradation of microtubule-associated proteins*
**作者**：Smith J, et al.
**摘要**：通过KLHL8抗体的免疫共沉淀实验，作者发现KLHL8与CUL3形成E3泛素连接酶复合体，靶向降解微管结合蛋白，参与细胞骨架动态调节。
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3. **文献名称**：*Tissue-specific expression of KLHL8 in neuronal development*
**作者**：Hernández L, et al.
**摘要**：利用KLHL8抗体的免疫组化分析，本文报道了KLHL8在神经元突触中的富集表达，提示其在神经发育和突触可塑性中的潜在作用。
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**注**：以上文献为示例性内容，实际文献需通过PubMed/Google Scholar等平台检索确认。如需具体文章，可提供更详细的研究背景或DOI号。
KLHL8 (Kelch-like family member 8) is a member of the Kelch-like protein family, characterized by its conserved BTB (Broad-Complex, Tramtrack, and Bric-à-brac) domain, BACK (BTB and C-terminal Kelch) domain, and Kelch repeat motifs. These structural features enable KLHL8 to function as a substrate-specific adaptor for Cullin3 (CUL3)-based E3 ubiquitin ligase complexes, facilitating the ubiquitination and subsequent proteasomal degradation of target proteins. KLHL8 is implicated in regulating cellular processes such as cytoskeletal organization, signal transduction, and protein quality control. Dysregulation of KLHL8 has been linked to various pathologies, including cancer, neurodegenerative disorders, and cardiovascular diseases, underscoring its potential role in disease mechanisms.
Antibodies targeting KLHL8 are critical tools for investigating its expression, localization, and functional interactions. These antibodies are commonly used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to study KLHL8's protein levels, binding partners, and tissue distribution. Given the structural homology among Kelch-like proteins, specificity validation (e.g., via knockout controls) is essential to ensure antibody reliability. Research utilizing KLHL8 antibodies has shed light on its involvement in ubiquitin-dependent pathways and disease contexts, making it a focus for therapeutic targeting and biomarker discovery. Current studies aim to clarify its substrate profiles and regulatory mechanisms in both physiological and pathological states.
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