| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | MARK4L; MARK4S; MARKL1; PAR-1D; MARKL1L |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human MARK4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于MARK4抗体的模拟参考文献示例(实际文献需通过学术数据库查询)：
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1. **文献名称**：*"Development of a Novel Monoclonal Antibody Targeting MARK4 for Alzheimer's Disease Research"*
**作者**：Smith A, et al.
**摘要**：本研究开发了一种高特异性抗MARK4单克隆抗体，验证了其在人脑组织中对MARK4蛋白的检测能力。研究发现MARK4在阿尔茨海默病患者大脑中异常表达，并与tau蛋白过度磷酸化显著相关，提示其作为治疗靶点的潜力。
2. **文献名称**：*"Structural Insights into MARK4 Kinase Domain and Antibody Interaction"*
**作者**：Chen L, et al.
**摘要**：通过X射线晶体学解析了MARK4激酶结构域与其特异性抗体的复合物结构，揭示了抗体结合表位及抑制MARK4活性的分子机制，为设计小分子抑制剂提供了结构基础。
3. **文献名称**：*"MARK4 Expression in Glioblastoma: A Prognostic Biomarker Study Using Immunohistochemistry"*
**作者**：Wang Y, et al.
**摘要**：利用新型兔源多克隆抗体分析胶质母细胞瘤组织中MARK4的表达水平，发现其高表达与患者生存期缩短及肿瘤侵袭性增强相关，提示其可作为预后生物标志物。
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**建议**：实际研究中，可通过PubMed或Web of Science以关键词“MARK4 antibody”、“MARK4 kinase”或“MARK4 disease association”检索最新文献，重点关注抗体开发、疾病机制或治疗应用方向。
MARK4 (microtubule affinity-regulating kinase 4), also known as PAR-1 homolog or C-TAK1. is a serine/threonine kinase belonging to the AMPK-related kinase family. It plays a critical role in regulating microtubule dynamics, cell polarity, and intracellular signaling pathways. Structurally, MARK4 contains a catalytic kinase domain, a ubiquitin-associated (UBA) domain, and a spacer region that modulates substrate interactions. Notably, it phosphorylates microtubule-associated proteins (e.g., tau, MAP2/4) to destabilize microtubules, influencing cell division, neuronal development, and organelle transport.
Research highlights its involvement in pathological conditions. Hyperphosphorylation of tau by MARK4 contributes to neurofibrillary tangle formation in Alzheimer’s disease. It also interacts with pathways like Wnt/β-catenin and mTOR, linking it to cancer progression, metabolic disorders, and autophagy dysregulation. MARK4 overexpression has been observed in several cancers, promoting metastasis and chemoresistance.
MARK4 antibodies are essential tools for studying its expression, localization, and function. They are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate MARK4's role in diseases. Recent studies explore MARK4 inhibitors as potential therapeutics, emphasizing the antibody's diagnostic and research relevance. Its dual role in neurodegeneration and oncology makes MARK4 a compelling target for interdisciplinary biomedical research.
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