| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | MLK3; PTK1; SPRK; MLK-3; MEKK11 |
| WB Predicted band size | 93 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human MAP3K11 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是3-4条关于MAP3K11抗体的参考文献示例(内容为虚构，供参考)：
1. **"MAP3K11 regulates JNK signaling in hepatocellular carcinoma"**
- 作者：Li et al.
- 摘要：研究通过Western blot和免疫组化验证了MAP3K11抗体在肝癌组织中的特异性，发现MAP3K11通过激活JNK通路促进肿瘤侵袭。
2. **"Role of MAP3K11 in adipocyte differentiation"**
- 作者：Smith et al.
- 摘要：利用MAP3K11抗体进行敲低实验，证明该激酶通过调控ERK1/2磷酸化抑制脂肪细胞分化。
3. **"MAP3K11 antibody validation for flow cytometry applications"**
- 作者：Garcia et al.
- 摘要：验证了多种MAP3K11抗体的交叉反应性，筛选出适用于流式细胞术的高特异性抗体，用于分析免疫细胞中的MAP3K11表达。
4. **"MAP3K11 mediates oxidative stress-induced neuronal apoptosis"**
- 作者：Wang et al.
- 摘要：通过siRNA和MAP3K11抗体阻断实验，揭示其在氧化应激中激活ASK1-JNK通路并诱导神经元凋亡的机制。
---
如需真实文献，建议在PubMed或Google Scholar检索关键词“MAP3K11 antibody”或“MAP3K11 + [应用领域]”。
The MAP3K11 antibody is a crucial tool for studying the mitogen-activated protein kinase kinase kinase 11 (MAP3K11), also known as MLK3 or PTK1. This serine/threonine kinase belongs to the MAP3K family and plays a pivotal role in signaling pathways regulating cellular responses to stress, apoptosis, inflammation, and proliferation. MAP3K11 activates downstream kinases like JNK and p38 MAPK through phosphorylation, often in response to cytokines, growth factors, or cellular damage. Structurally, it contains a kinase domain, a leucine zipper motif, and an SH3-binding motif, enabling interactions with adaptor proteins and scaffolding complexes.
Antibodies targeting MAP3K11 are widely used in Western blotting, immunoprecipitation, and immunohistochemistry to detect protein expression, localization, and activation states in tissues or cultured cells. They are essential for investigating MAP3K11's involvement in diseases such as cancer, neurodegenerative disorders, and metabolic syndromes, where its dysregulation is linked to tumor progression, insulin resistance, or neuronal death. Validated antibodies typically undergo specificity checks using knockout cell lines or siRNA-mediated knockdown. Researchers also utilize these antibodies to explore therapeutic targeting of MAP3K11. as its inhibition shows potential in modulating pathological signaling cascades. Host species (e.g., rabbit, mouse) and clonality (monoclonal/polyclonal) vary, allowing flexibility in experimental design.
×
