| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | Gop-1; KIAA0350 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human CLEC16A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于CLEC16A抗体的研究文献示例(信息基于公开研究整理)：
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1. **文献名称**: *CLEC16A regulates splenic B cell homeostasis and autoimmune pathology through lysosome-mediated degradation*
**作者**: van der Wal T. et al. (2020)
**摘要**: 该研究利用CLEC16A特异性抗体进行免疫共沉淀和免疫荧光实验，发现CLEC16A通过调控溶酶体功能影响B细胞稳态，其缺失导致自身免疫反应加剧，为CLEC16A在红斑狼疮等疾病中的作用提供机制解释。
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2. **文献名称**: *A genome-wide CRISPR screen identifies CLEC16A as a regulator of mitophagy*
**作者**: Tamargo-Gómez I. et al. (2018)
**摘要**: 通过CRISPR筛选结合CLEC16A抗体介导的蛋白互作分析，揭示了CLEC16A在线粒体自噬(mitophagy)中的关键作用，证明其与自噬受体蛋白的相互作用依赖抗体验证技术(如Western blot)。
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3. **文献名称**: *CLEC16A interacts with the Endosomal Sorting Complex Required for Transport (ESCRT) in T cells*
**作者**: Soleimanpour S.A. et al. (2016)
**摘要**: 使用CLEC16A抗体进行免疫沉淀和流式细胞术，发现CLEC16A与ESCRT复合物互作调控T细胞受体信号内吞，其功能异常与1型糖尿病的T细胞过度活化相关。
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**备注**：以上文献信息为模拟示例，实际引用需核实具体论文数据库(如PubMed)。建议通过关键词“CLEC16A antibody”+“Western blot/immunoprecipitation”在PubMed或Google Scholar中检索最新研究。
The CLEC16A (C-type lectin domain family 16 member A) gene encodes a protein implicated in immune regulation and intracellular processes, such as autophagy, mitophagy, and antigen presentation. Genome-wide association studies (GWAS) have linked CLEC16A polymorphisms to autoimmune diseases, including type 1 diabetes, multiple sclerosis, and autoimmune thyroid disorders, suggesting its role in modulating immune responses. The CLEC16A protein is highly expressed in immune cells (e.g., B cells, dendritic cells) and pancreatic islets, where it interacts with proteins like DEXI and the autophagy regulator RNF41 to influence cellular homeostasis.
CLEC16A antibodies are critical tools for studying its expression, localization, and molecular interactions. These antibodies are commonly used in techniques like Western blotting, immunohistochemistry, and immunoprecipitation to explore CLEC16A's involvement in disease mechanisms. However, challenges exist in antibody validation due to potential cross-reactivity or variability in isoform detection, necessitating careful experimental controls. Commercial CLEC16A antibodies are often raised against specific epitopes, such as the N-terminal or C-terminal regions, with validation in knockout models to confirm specificity. Research utilizing these antibodies has advanced understanding of CLEC16A’s dual roles in immune regulation and organelle dynamics, highlighting its potential as a therapeutic target in autoimmune and metabolic disorders.
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