Rabbit Polyclonal CYBB Antibody

The CYBB gene encodes gp91phox, a critical subunit of the NADPH oxidase complex (NOX2) responsible for generating reactive oxygen species (ROS) in phagocytes to eliminate pathogens. Antibodies targeting CYBB/gp91phox are essential tools in studying immune function, particularly in chronic granulomatous disease (CGD), an inherited disorder caused by mutations in CYBB that impair ROS production. These antibodies enable detection of gp91phox expression via techniques like Western blot, immunohistochemistry, and flow cytometry, aiding in the assessment of NADPH oxidase assembly and activity. Commercially available CYBB antibodies, often raised against synthetic peptides or recombinant protein fragments, are validated for specificity using knockout cell lines or CGD patient samples. Research applications extend to investigating oxidative stress-related pathways, immune cell activation, and inflammatory diseases. Additionally, CYBB antibodies support therapeutic development, such as monitoring gene therapy outcomes in CGD or evaluating NOX2's role in non-infectious conditions like autoimmune disorders. Their utility in both diagnostic and mechanistic studies underscores their importance in bridging basic immunology and clinical translation.