| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | MOX-2; GP91-3 |
| WB Predicted band size | 65 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human NOX3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于NOX3抗体的3篇模拟参考文献示例(请注意文献信息为模拟生成，建议通过学术数据库核实)：
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1. **文献名称**: *"NOX3-Dependent Oxidative Stress in the Inner Ear: Localization and Functional Implications"*
**作者**: Sorce, S., Krause, K.H.
**摘要**: 本研究利用特异性NOX3抗体，通过免疫组化技术揭示了NOX3在内耳毛细胞中的高表达，并证实其通过产生活性氧(ROS)参与听觉信号传导及氧化损伤机制。
2. **文献名称**: *"Role of NOX3 in Ovarian Cancer Progression: Insights from Antibody-Based Inhibition Studies"*
**作者**: Matsushima, Y., et al.
**摘要**: 通过抗NOX3抗体阻断实验，研究发现NOX3在卵巢癌细胞中高表达，并通过ROS介导的MAPK通路促进肿瘤增殖和转移，提示其作为潜在治疗靶点。
3. **文献名称**: *"Development and Validation of a Novel Monoclonal Antibody for Human NOX3 Detection"*
**作者**: Ueno, N., et al.
**摘要**: 本文报道了一种高特异性抗人NOX3单克隆抗体的制备，经Western blot和免疫荧光验证，成功应用于检测肾脏和神经组织中的NOX3蛋白表达水平。
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**备注**：若需真实文献，建议通过PubMed或Web of Science检索关键词“NOX3 antibody”或“NADPH oxidase 3”，并筛选涉及抗体开发、定位或功能研究的文章。
NOX3 (NADPH oxidase 3) is a member of the NOX family of enzymes responsible for generating reactive oxygen species (ROS) through the reduction of molecular oxygen. Primarily expressed in the inner ear, particularly the cochlea and vestibular system, NOX3 plays a critical role in auditory function and balance regulation. Unlike other NOX isoforms, NOX3 exhibits tissue-specific activity, with its expression tightly linked to the development and maintenance of otoconia—calcium carbonate crystals essential for gravity sensing. Dysregulation of NOX3 has been implicated in oxidative stress-related pathologies, including noise-induced hearing loss (NIHL), age-related hearing impairment, and vestibular dysfunction. Its ROS production is also associated with inflammatory signaling and cellular damage in other tissues under specific conditions.
NOX3 antibodies are vital tools for studying its expression, localization, and function in both physiological and pathological contexts. They enable detection via techniques like Western blotting, immunohistochemistry, and immunofluorescence, aiding research into NOX3's interaction with regulatory subunits (e.g., p22phox, NOXO1) and its role in redox-sensitive pathways. Recent studies explore NOX3 as a therapeutic target, with inhibitors and gene-silencing approaches being tested to mitigate ROS-mediated damage. However, challenges remain in understanding its precise regulatory mechanisms and tissue-specific activation. The development of high-specificity NOX3 antibodies continues to advance insights into its contributions to hearing disorders and broader oxidative stress diseases, highlighting its potential in translational research.
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