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产品描述：人脱氧核糖核酸酶Ⅰ(DNase-Ⅰ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的DNASE1含量。DNASE1，即脱氧核糖核酸酶I，是一种能够消化单链或双链DNA的非特异性核酸内切酶。它通过水解DNA的磷酸二酯键，产生单脱氧核苷酸或寡脱氧核苷酸。DNASE1在RNA提取、DNA-蛋白质相互作用研究以及基因编辑等领域有广泛应用。试剂盒检测范围为1.56 ng/mL-100 ng/mL，适用于科研领域中对DNase - 1的定量分析，例如探究其在疾病发生发展中的分子机制、评估药物干预对酶活性的影响，或作为细胞凋亡相关研究的辅助工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Deoxyribonuclease 1 ELISA Kit; Deoxyribonuclease I ELISA Kit; Deoxyribonuclease-1 ELISA Kit; Deoxyribonuclease1 ELISA Kit; DeoxyribonucleaseI ELISA Kit; DNAS1_HUMAN ELISA Kit; DNASE 1 ELISA Kit; DNase I ELISA Kit; DNase I lysosomal ELISA Kit; DNASE1 ELISA Kit; DNaseI ELISA Kit; DNL 1 ELISA Kit; DNL1 ELISA Kit; Dornase alfa ELISA Kit; DRNI ELISA Kit; FLJ38093 ELISA Kit; Human urine deoxyribonuclease I ELISA Kit
缩写：DNASE1
Uniprot No.：P24855
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human DNase-Ⅰ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 83-92 | |||||
| 1:2 | Average % | 110 | ||||
| Range % | 105-115 | |||||
| 1:4 | Average % | 85 | ||||
| Range % | 81-90 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of human DNase-Ⅰ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 87 | 85-90 | ||||
| EDTA plasma (n=4) | 95 | 89-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected1002.5622.5982.5802.435502.0732.0982.0861.941251.3141.3651.3401.19512.50.6950.6680.6820.5376.250.4320.4510.4420.2973.120.2870.2680.2780.1331.560.2040.2090.2070.06200.1430.1460.145 | ||||||
货期：3-5 working days
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