| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/1000-1/2000 | Human,Mouse,Rat |
| Aliases | SAX3 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human ROBO2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ROBO2抗体的3篇参考文献的简要信息，基于模拟生成内容整理：
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1. **文献名称**: *Monoclonal Antibody Targeting ROBO2 Inhibits Tumor Angiogenesis in Colorectal Cancer Models*
**作者**: Smith A, et al.
**摘要**: 本研究开发了一种靶向ROBO2的单克隆抗体，通过阻断Slit-ROBO2信号通路抑制肿瘤血管生成。实验表明，该抗体显著降低结直肠癌小鼠模型的肿瘤生长和转移，提示其在抗肿瘤治疗中的潜在应用。
2. **文献名称**: *ROBO2-Specific Antibody Reveals Its Role in Murine Kidney Development*
**作者**: Johnson R, et al.
**摘要**: 利用新型ROBO2特异性抗体进行免疫组化和免疫荧光分析，揭示ROBO2在胚胎小鼠肾脏发育中的时空表达模式。抗体阻断实验证明ROBO2对肾小管分支形态发生的关键作用。
3. **文献名称**: *Structural Characterization of ROBO2 Epitopes for Therapeutic Antibody Design*
**作者**: Lee H, et al.
**摘要**: 通过X射线晶体学解析ROBO2胞外域与候选治疗性抗体的复合物结构，确定其抗原结合表位。该研究为优化靶向ROBO2的抗体药物(如用于神经母细胞瘤治疗)提供了分子基础。
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注：以上文献为示例性质，实际引用请根据具体研究通过学术数据库(如PubMed、Web of Science)核实。
The ROBO2 (Roundabout Homolog 2) antibody is a tool used to detect and study the ROBO2 protein, a transmembrane receptor critical in the Slit-Robo signaling pathway. ROBO2. part of the immunoglobulin (Ig) superfamily, interacts with Slit ligands to regulate cell migration, axon guidance, and tissue morphogenesis during development. It plays a key role in neural circuit formation, vascular patterning, and organogenesis, particularly in the urinary system. Dysregulation of ROBO2 is linked to congenital anomalies like vesicoureteral reflux (VUR) and kidney malformations, as well as cancers where it may influence metastasis or proliferation.
ROBO2 antibodies are essential in research to visualize protein expression, localization, and interactions via techniques like immunohistochemistry, Western blotting, or immunofluorescence. They help elucidate ROBO2’s role in developmental disorders and cancer progression. Commercial ROBO2 antibodies are typically raised against specific epitopes, often in extracellular Ig or fibronectin domains. Validation for specificity (e.g., knockout controls) is crucial due to potential cross-reactivity with homologous proteins like ROBO1. Studies using these antibodies have expanded understanding of Slit-Robo signaling in health and disease, highlighting therapeutic targeting potential.
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