| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | RSK; HU-2; RSK3; p90-RSK3; pp90RSK3; MAPKAPK1C; S6K-alpha; S6K-alpha2 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human RPS6KA2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于RPS6KA2抗体的参考文献摘要信息：
1. **文献名称**: "RPS6KA2 regulates tumor progression in colorectal cancer through mTOR pathway"
**作者**: Li, X., et al.
**摘要**: 该研究利用RPS6KA2特异性抗体进行免疫印迹和免疫组化分析，发现RPS6KA2通过激活mTOR信号通路促进结直肠癌细胞增殖和转移，抗体验证了其在肿瘤组织中的高表达。
2. **文献名称**: "Functional characterization of RPS6KA2 in neuronal differentiation using CRISPR/Cas9 and antibody-based assays"
**作者**: Martinez, R., & Kim, S.
**摘要**: 作者通过RPS6KA2抗体进行免疫荧光染色，证明RPS6KA2在神经干细胞分化中调控MAPK/ERK通路，抗体特异性经siRNA敲低实验验证。
3. **文献名称**: "RPS6KA2 as a potential biomarker in breast cancer: Validation by immunohistochemistry"
**作者**: Gupta, P., et al.
**摘要**: 研究使用商业化RPS6KA2抗体对乳腺癌组织样本进行免疫组化分析，发现其表达水平与患者预后负相关，抗体特异性通过质谱分析确认。
注：以上文献为虚拟示例，实际文献需通过PubMed或Google Scholar检索关键词“RPS6KA2 antibody”、“MSK1 antibody”获取。
The RPS6KA2 antibody targets the ribosomal protein S6 kinase alpha-2 (RPS6KA2), a serine/threonine kinase belonging to the p90 ribosomal S6 kinase (RSK) family. This protein is encoded by the RPS6KA2 gene located on human chromosome 6q27 and plays a critical role in the MAPK/ERK signaling pathway. RPS6KA2 is activated by phosphorylation in response to growth factors, hormones, or stress signals, mediating cellular processes such as proliferation, differentiation, and survival. Structurally, it contains two kinase domains (N-terminal and C-terminal) regulated by coordinated phosphorylation events.
RPS6KA2 is highly expressed in the brain, particularly in regions associated with cognition and neurodevelopment, suggesting its involvement in synaptic plasticity and neuronal signaling. Dysregulation of RPS6KA2 has been linked to neurodevelopmental disorders, including X-linked intellectual disability and autism spectrum disorders. Antibodies against RPS6KA2 are essential tools in biomedical research, enabling detection of protein expression, phosphorylation status, and localization via techniques like Western blotting, immunohistochemistry, and immunofluorescence. They are widely used to study its role in neurological diseases, cancer (e.g., breast and ovarian cancers), and its interaction with downstream substrates like CREB. Validation of these antibodies often includes knockout controls to ensure specificity, given the structural similarities among RSK family members.
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