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产品描述：人脱氧核糖核酸酶γ(DNASE1L3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的DNASE1L3含量。DNASE1L3 是一种重要靶点。它属于脱氧核糖核酸酶家族，在维持核酸代谢平衡等方面有一定作用。研究机制方面，其主要通过降解特定核酸来发挥功能，异常表达或活性改变可能与自身免疫性疾病等病理过程存在关联。试剂盒检测范围为23.44 pg/mL-1500 pg/mL，适用于基础医学研究中探索DNASE1L3在疾病模型中的表达调控、评估药物干预对核酸酶活性的影响，或用于体外培养细胞凋亡过程中相关分子机制的研究，为免疫学、肿瘤学及细胞生物学领域提供可靠的实验工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Deoxyribonuclease gamma ELISA Kit; Deoxyribonuclease I like 3 ELISA Kit; Deoxyribonuclease I like III ELISA Kit; Deoxyribonuclease I-like 3 ELISA Kit; DHP 2 ELISA Kit; DHP2 ELISA Kit; DNAS1L3 ELISA Kit; DNase gamma ELISA Kit; DNase I homolog protein 2 ELISA Kit; DNase I homolog protein DHP2 ELISA Kit; DNase I like 3 ELISA Kit; DNase I-like 3 ELISA Kit; DNASE1L3 ELISA Kit; DNSL3_HUMAN ELISA Kit; Liver and spleen DNase ELISA Kit; LS DNase ELISA Kit; LS-DNase ELISA Kit; LSD ELISA Kit; SLEB 16 ELISA Kit; SLEB16 ELISA Kit
缩写：DNASE1L3
Uniprot No.：Q13609
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：23.44 pg/mL-1500 pg/mL
灵敏度：5.86 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human DNASE1L3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 91 | |||||
| Range % | 86-95 | ||||||
| 1:2 | Average % | 102 | |||||
| Range % | 97-107 | ||||||
| 1:4 | Average % | 91 | |||||
| Range % | 85-97 | ||||||
| 1:8 | Average % | 97 | |||||
| Range % | 91-103 | ||||||
回收率：
| The recovery of human DNASE1L3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 89-98 | |||||
| EDTA plasma (n=4) | 97 | 90-100 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected15002.6342.5382.5862.5007502.2232.3022.2632.1773751.8171.8351.8261.740187.51.1171.2041.1611.07593.750.7590.7480.7540.66846.880.4840.4980.4910.40523.440.2770.2610.2690.18300.0870.0850.086 | |||||||
货期：3-5 working days
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