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产品描述：人dopey-2蛋白(DOPEY2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的DOPEY2含量。DOPEY2是一种蛋白质编码基因，与认知和胚胎模式指定有关，位于早期内体膜上。其可能参与调节货物蛋白的膜运输，与ATP9A和MON2一起调节SNX3逆反器介导的内体排序，避免WLS被溶酶体降解。DOPEY2的研究涉及其在细胞糖基化和囊泡运输中的影响，以及其在胚胎神经发生中与Pcdh7协同调控神经干细胞增殖和分化的作用。试剂盒检测范围为32 pg/mL-1200 pg/mL，支持科研工作者在神经生物学、细胞代谢调控或疾病机制研究中开展定量分析；适用于脑组织、外周血样本或体外培养细胞模型中DOPEY2表达水平的高通量检测，为探究其在神经退行性疾病、代谢综合征等病理过程中的作用提供数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：DOP1B ELISA Kit; C21orf5 ELISA Kit; DOPEY2 ELISA Kit; KIAA0933Protein dopey-2 ELISA Kit
缩写：DOPEY2
Uniprot No.：Q9Y3R5
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：32 pg/mL-1200 pg/mL
灵敏度：8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human DOPEY2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-97 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-103 | |||||
| 1:4 | Average % | 85 | ||||
| Range % | 81-89 | |||||
| 1:8 | Average % | 89 | ||||
| Range % | 83-95 | |||||
回收率：
| The recovery of human DOPEY2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 104 | 102-106 | ||||
| EDTA plasma (n=4) | 93 | 90-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected12002.3132.4512.3822.3356401.7541.8021.7781.7313201.0121.0321.0220.975800.3450.3550.3500.303320.1730.1740.1740.12700.0450.0480.047 | ||||||
货期：3-5 working days
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