Mouse Elastin(ELN) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-EL007617MO

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：
小鼠弹性蛋白(ELN)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的ELN含量。试剂盒检测范围为3.12 ng/mL-200 ng/mL，可应用于心血管疾病模型、皮肤组织修复研究或生物材料弹性评估等场景，支持科研人员探究ELN在组织发育、病理机制中的调控作用本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：
ElnElastin ELISA Kit; Tropoelastin ELISA Kit
缩写：
ELN
Uniprot No.：
P54320
种属：
Mus musculus (Mouse)
样本类型：
serum, plasma, tissue homogenates
检测范围：
3.12 ng/mL-200 ng/mL
灵敏度：
0.78 ng/mL
反应时间：
1-5h
样本体积：
50-100ul
检测波长：
450 nm
研究领域：
Signal Transduction
测定原理：
quantitative
测定方法：
Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ELN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	96
	Range %	91-101
1:2	Average %	100
	Range %	95-105
1:4	Average %	108
	Range %	101-114
1:8	Average %	92
	Range %	86-98

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


ng/ml	OD1	OD2	Average	Corrected
200	2.804	2.744	2.774	2.622
100	2.127	2.067	2.097	1.945
50	1.441	1.408	1.425	1.273
25	0.863	0.829	0.846	0.694
12.5	0.527	0.521	0.524	0.372
6.25	0.394	0.403	0.399	0.247
3.12	0.258	0.264	0.261	0.109
0	0.149	0.155	0.152