| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/35-1/150 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | HI; SUR; HHF1; MRP8; PHHI; SUR1; ABC36; HRINS; TNDM2; SUR1delta2 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Rat |
| Immunogen | Synthetic peptide of human ABCC8 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ABCC8抗体的3篇代表性文献的模拟示例(非真实文献，仅供格式参考)：
1. **文献名称**: "ABCC8/SUR1 Expression in Congenital Hyperinsulinism: Immunohistochemical Analysis"
**作者**: Smith A, et al.
**摘要**: 通过免疫组织化学技术研究ABCC8编码的SUR1蛋白在先天性高胰岛素血症患者胰腺组织中的表达模式，发现ABCC8抗体特异性标记β细胞膜，并与特定突变导致的通道功能缺陷相关。
2. **文献名称**: "Functional Characterization of ABCC8 Mutations Using Western Blot and Antibody-Based Assays"
**作者**: Zhang L, et al.
**摘要**: 利用ABCC8特异性抗体进行Western blot和免疫荧光实验，分析不同突变对SUR1蛋白稳定性及细胞定位的影响，揭示部分突变导致蛋白降解加速的分子机制。
3. **文献名称**: "Development of a Novel ABCC8 Antibody for Diabetes Research"
**作者**: Gupta R, et al.
**摘要**: 报道一种高灵敏度兔源多克隆抗体的开发，该抗体通过ELISA和流式细胞术验证，可用于检测人/鼠ABCC8蛋白表达水平，为糖尿病模型研究提供工具。
4. **文献名称**: "ABCC8 Antibody Reveals Altered SUR1 Localization in Insulinoma Cells"
**作者**: Tanaka K, et al.
**摘要**: 使用ABCC8抗体结合共聚焦显微镜，发现胰岛素瘤细胞中SUR1蛋白异常聚集于内质网，提示其转运障碍可能与肿瘤发生相关。
(注：以上文献为模拟内容，实际文献需通过PubMed/Google Scholar等平台检索关键词“ABCC8 antibody”“SUR1 immunohistochemistry”等获取。)
The ABCC8 antibody targets the protein product of the ATP-binding cassette subfamily C member 8 (ABCC8) gene, which encodes sulfonylurea receptor 1 (SUR1). SUR1 is a regulatory subunit of ATP-sensitive potassium (KATP) channels, predominantly expressed in pancreatic β-cells, neurons, and other tissues. These channels link cellular metabolic status to membrane excitability, playing a critical role in insulin secretion regulation. ABCC8 mutations are associated with congenital hyperinsulinism, neonatal diabetes, and neurological disorders like DEND syndrome.
ABCC8 antibodies are essential tools for studying SUR1 expression, localization, and function in health and disease. They are widely used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to investigate KATP channel dynamics, β-cell dysfunction in diabetes, and drug mechanisms (e.g., sulfonylureas, which bind SUR1 to stimulate insulin secretion). These antibodies also aid in diagnosing genetic disorders linked to ABCC8 variants.
Challenges in ABCC8 antibody development include SUR1's complex transmembrane structure and homology with other ABC transporters. Specificity validation via knockout controls is crucial to avoid cross-reactivity. Commercial antibodies often target epitopes in cytoplasmic domains (e.g., nucleotide-binding domains) or extracellular loops, with varying cross-species reactivity (human, mouse, rat). Advances in recombinant antibody technology continue to improve their precision for research and clinical applications.
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