首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人Epigen(EPGN)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的EPGN含量。EPGN 即表皮生长因子样蛋白，在多种肿瘤中异常表达。其作为细胞膜生长因子，可激活下游信号通路促进细胞增殖、迁移等。目前围绕 EPGN 的研究多聚焦于其在肿瘤发生发展中的机制，有望为肿瘤诊断和治疗提供新靶点和思路。试剂盒检测范围为1.56 ng/mL-100 ng/mL，本试剂盒适用于体外研究EPGN在肿瘤生物学、细胞信号通路或炎症相关分子机制中的表达特征，支持多种样本类型检测，具有高灵敏度和特异性，可满足基础科研中对EPGN蛋白表达动态变化的精确分析需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：ALGV3072 ELISA Kit; EPG ELISA Kit; EPGN ELISA Kit; EPGN_HUMAN ELISA Kit; Epigen ELISA Kit; Epigen precursor ELISA Kit; Epithelial mitogen ELISA Kit; PRO9904 ELISA Kit
缩写：EPGN
Uniprot No.：Q6UW88
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human EPGN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 103 | |||||
| Range % | 96-107 | ||||||
| 1:2 | Average % | 102 | |||||
| Range % | 97-105 | ||||||
| 1:4 | Average % | 92 | |||||
| Range % | 85-96 | ||||||
| 1:8 | Average % | 97 | |||||
| Range % | 92-101 | ||||||
回收率：
| The recovery of human EPGN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 97 | 92-103 | |||||
| EDTA plasma (n=4) | 105 | 98-109 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected 1002.6872.5682.6282.528 502.1432.0022.0731.973 251.2981.2771.2881.188 12.50.7320.7050.7190.619 6.250.4140.3930.4040.304 3.120.2630.2520.2580.158 1.560.1720.1680.1700.070 00.1010.0990.100 | |||||||
货期：3-5 working days
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