| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | LIN41; LIN-41 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human TRIM71 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇涉及TRIM71抗体的代表性文献摘要：
1. **"TRIM71 cooperates with microRNAs to repress Cdkn1a and promote embryonic stem cell proliferation"**
*作者：Chang H et al. (2012)*
摘要：研究利用TRIM71特异性抗体进行免疫沉淀和Western blot，揭示TRIM71通过结合miRNA复合物调控细胞周期蛋白Cdkn1a，促进胚胎干细胞增殖的机制。
2. **"The RNA-binding protein TRIM71 regulates cell cycle by targeting p21 in hepatocellular carcinoma"**
*作者：Li Y et al. (2018)*
摘要：通过TRIM71抗体进行免疫组化分析临床样本，发现TRIM71在肝癌中高表达，且通过抑制p21加速细胞周期进程，提示其作为潜在治疗靶点。
3. **"TRIM71 interacts with Argonaute2 to regulate epidermal stem cell fate"**
*作者：Chen J et al. (2020)*
摘要：研究使用TRIM71抗体进行共免疫沉淀和免疫荧光，证明TRIM71与Argonaute2互作，调控表皮干细胞分化，影响皮肤组织稳态。
注：以上文献信息为示例，实际引用需以具体论文数据为准。建议通过PubMed或Google Scholar以关键词“TRIM71 antibody”+"validation"/"application"检索最新研究。
TRIM71 (Tripartite Motif-Containing 71), also known as LIN-41. is a member of the TRIM protein family characterized by conserved RING, B-box, and coiled-coil domains. It functions as an RNA-binding protein and E3 ubiquitin ligase, playing critical roles in post-transcriptional gene regulation, embryonic development, and cellular differentiation. TRIM71 is notably involved in stem cell maintenance and reprogramming by targeting specific mRNAs for degradation or translational repression, often interacting with microRNAs (miRNAs) and components of the RNA-induced silencing complex (RISC). Its activity is crucial during early embryogenesis, particularly in regulating the transition from pluripotency to differentiation.
In cancer biology, TRIM71 exhibits dual roles as either an oncogene or tumor suppressor, depending on cellular context. Overexpression has been linked to tumor progression in certain cancers (e.g., hepatocellular carcinoma, glioblastoma) by promoting cell proliferation and inhibiting apoptosis, while its downregulation is associated with poor prognosis in others. These context-dependent functions make TRIM71 a compelling therapeutic target and biomarker candidate.
TRIM71 antibodies are essential tools for investigating its expression patterns, subcellular localization, and molecular interactions. They are widely used in techniques like Western blotting, immunohistochemistry, and immunoprecipitation to study TRIM71's role in development, tissue homeostasis, and disease. Recent research also explores its potential in regenerative medicine and targeted cancer therapies. Validation of TRIM71 antibodies remains critical due to homology with other TRIM family members and the protein's complex post-translational modifications.
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