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产品描述：人低氧诱导因子2(HIF-2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的EPAS1含量。EPAS1即内皮PAS结构域蛋白1，在低氧适应等生理过程中起重要作用。其研究机制主要围绕它作为低氧诱导因子，能调控下游基因的表达，参与血管生成、能量代谢等多个生理和病理过程，对肿瘤、心血管疾病等研究有重要意义。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于体外培养细胞低氧模型机制研究、动物疾病模型中HIF - 2动态变化监测，以及相关信号通路调控药物的体外筛选等科研场景。可为心血管疾病、肿瘤微环境、高原生理适应等领域的分子机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Basic helix loop helix PAS protein MOP2 ELISA Kit; Basic-helix-loop-helix-PAS protein MOP2 ELISA Kit; bHLHe73 ELISA Kit; Class E basic helix-loop-helix protein 73 ELISA Kit; ECYT4 ELISA Kit; Endothelial PAS domain containing protein 1 ELISA Kit; Endothelial pas domain protein 1 ELISA Kit; Endothelial PAS domain-containing protein 1 ELISA Kit; EPAS 1 ELISA Kit; EPAS-1 ELISA Kit; EPAS1 ELISA Kit; EPAS1_HUMAN ELISA Kit; HIF 1 alpha like factor ELISA Kit; HIF 2 alpha ELISA Kit; HIF-1-alpha-like factor ELISA Kit; HIF-2-alpha ELISA Kit; HIF2-alpha ELISA Kit; HIF2A ELISA Kit; HLF ELISA Kit; Hypoxia inducible factor 2 alpha ELISA Kit; Hypoxia inducible factor 2 alpha subunit ELISA Kit; Hypoxia-inducible factor 2-alpha ELISA Kit; Member of PAS protein 2 ELISA Kit; Member of pas superfamily 2 ELISA Kit; MOP 2 ELISA Kit; MOP2 ELISA Kit; PAS domain-containing protein 2 ELISA Kit; PASD2 ELISA Kit
缩写：EPAS1
Uniprot No.：Q99814
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human HIF-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 82-93 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 94-100 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 90-104 | |||||
| 1:8 | Average % | 85 | ||||
| Range % | 82-93 | |||||
回收率：
| The recovery of human HIF-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 87-95 | ||||
| EDTA plasma (n=4) | 101 | 96-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected40002.7602.7402.7502.60520002.1842.3502.2672.12210001.4391.5201.4801.3355000.7120.7300.7210.5762500.4450.4310.4380.2931250.3520.3420.3470.20262.50.2510.2650.2580.11300.1440.1450.145 | ||||||
货期：3-5 working days
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