| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | LI; KTG; LI1; TGK; ICR2; ARCI1; TGASE |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human TGM1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于TGM1抗体的3篇代表性文献摘要(文献信息为示例性质，具体内容需根据实际文献调整)：
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1. **文献名称**：*Mutations in TGM1 as a cause of autosomal recessive congenital ichthyosis*
**作者**：Huber M, et al.
**摘要**：该研究通过基因测序和免疫组化分析，发现TGM1基因突变导致转谷氨酰胺酶1功能缺失，引发皮肤角化异常。研究利用特异性TGM1抗体证实患者皮肤样本中酶活性降低，为先天性鱼鳞病的分子诊断提供了依据。
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2. **文献名称**：*Transglutaminase 1 enzyme activity in epidermal differentiation*
**作者**：Nemes Z, et al.
**摘要**：本文通过TGM1抗体结合酶活性检测，揭示转谷氨酰胺酶1在表皮角质细胞终末分化中的关键作用，并证明其通过交联角蛋白包膜蛋白形成皮肤屏障功能，缺陷可能导致鳞屑性疾病。
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3. **文献名称**：*Immunohistochemical localization of TGM1 in normal and pathologic skin*
**作者**：Kalinin AE, et al.
**摘要**：研究利用TGM1特异性抗体进行皮肤组织染色，发现其在颗粒层和角质层高表达，并在鱼鳞病患者中呈现异常分布，表明TGM1抗体可作为皮肤病理诊断工具。
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4. **文献名称**：*Diagnostic value of TGM1 antibody in congenital ichthyosis subtypes*
**作者**：Oji V, et al.
**摘要**：通过比较不同鱼鳞病亚型患者的TGM1抗体检测结果，发现其敏感性及特异性较高，尤其适用于隐性遗传病例的快速筛查，为临床分型提供支持。
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注：以上文献为示例性概括，实际引用需核实具体发表信息及内容。建议通过PubMed或学术数据库检索最新研究。
**Background of TGM1 Antibody**
Transglutaminase 1 (TGM1), a calcium-dependent enzyme encoded by the *TGM1* gene, plays a critical role in epidermal differentiation and skin barrier formation. It catalyzes cross-linking of structural proteins, such as involucrin and loricrin, to form the cornified cell envelope, essential for maintaining skin integrity and hydration. Mutations in *TGM1* are linked to autosomal recessive congenital ichthyosis (ARCI), particularly lamellar ichthyosis, characterized by severe scaling and impaired barrier function.
TGM1 antibodies are immunological tools designed to detect and quantify TGM1 protein expression in research and diagnostics. These antibodies are pivotal in studying epidermal biology, elucidating mechanisms of skin disorders, and validating TGM1 deficiency in genetic studies. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to localize TGM1 in tissues or assess its expression levels.
Additionally, TGM1 antibodies hold therapeutic potential. In gene therapy research for ARCI, they help monitor TGM1 restoration in preclinical models. Recent advances also explore their use in enzyme replacement strategies or as targeting agents in drug delivery systems. However, challenges remain in ensuring antibody specificity and minimizing cross-reactivity with other transglutaminase isoforms (e.g., TGM2 or TGM3). Overall, TGM1 antibodies serve as indispensable reagents in dermatological research, bridging molecular insights to clinical applications.
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