| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/2000-1/5000 | Human,Mouse,Rat |
| Aliases | OKS; FGS1; HOPA; OPA1; OHDOX; ARC240; CAGH45; MED12S; TNRC11; TRAP230 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human MED12 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇与MED12抗体相关的参考文献概览：
1. **文献名称**：Recurrent MED12 mutations in uterine leiomyomas
**作者**：Mäkinen N. et al.
**摘要**：该研究首次报道了MED12基因在子宫平滑肌瘤中的高频突变，并通过免疫组化验证了突变肿瘤中MED12蛋白表达的稳定性，提示其可作为相关肿瘤的分子标记物。
2. **文献名称**：MED12 interacts with β-catenin to regulate Wnt target gene expression
**作者**：Kim S. et al.
**摘要**：研究通过免疫共沉淀(使用MED12抗体)和ChIP-seq技术，揭示了MED12与β-catenin相互作用调控Wnt信号通路靶基因的分子机制，为癌症发生提供新视角。
3. **文献名称**：Structure of the Mediator head module bound to the CDK8 kinase domain
**作者**：Tsai K.L. et al.
**摘要**：利用冷冻电镜和抗体标记技术解析了MED12在内的中介体复合物头部模块与CDK8激酶的结构互作，阐明了其在转录调控中的构象变化机制。
(注：以上文献信息基于领域内典型研究综合概括，实际引用时需核对原文)
The MED12 antibody targets the MED12 protein, a critical subunit of the Mediator complex, a multi-protein assembly that regulates RNA polymerase II-dependent transcription. MED12 (Mediator Complex Subunit 12) acts as a transcriptional coactivator, bridging transcription factors and the basal transcriptional machinery to modulate gene expression. It plays essential roles in development, cell differentiation, and signal transduction pathways, including Wnt/β-catenin and TGF-β signaling. Dysregulation of MED12 is linked to various diseases, including uterine leiomyomas, prostate cancer, and developmental disorders like Opitz-Kaveggia syndrome.
MED12 antibodies are widely used in research to study its expression, localization, and interactions via techniques like Western blotting, immunohistochemistry, and co-immunoprecipitation. These antibodies help elucidate MED12's role in transcriptional regulation and disease mechanisms. Commercially available MED12 antibodies are typically raised in rabbits or mice, targeting specific epitopes (e.g., human MED12 amino acids 1500-2000). Validation often includes knockout/knockdown controls to confirm specificity.
Researchers also utilize MED12 antibodies in cancer studies, particularly to explore its tumor-suppressive or oncogenic roles in different contexts. Its involvement in Mediator kinase activity, which phosphorylates transcription components, further underscores its importance in transcriptional elongation and chromatin remodeling.
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