| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | EK7; CEK7; EHK1; HEK7; EHK-1; TYRO4 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human EPHA5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于EPHA5抗体的示例参考文献(模拟生成，仅供参考)：
1. **文献名称**：*EPHA5 receptor modulates synaptic plasticity and cognitive decline in Alzheimer's disease models*
**作者**：Chen L, et al.
**摘要**：研究通过EPHA5抗体检测发现，EPHA5在阿尔茨海默病小鼠模型中异常表达，其与突触功能损伤和认知衰退相关，抑制EPHA5信号可改善病理表型。
2. **文献名称**：*EPHA5 as a prognostic biomarker in glioblastoma: Insights from antibody-based tissue analysis*
**作者**：Wang Y, et al.
**摘要**：利用EPHA5抗体对胶质母细胞瘤组织进行免疫组化分析，发现EPHA5高表达与患者生存期缩短显著相关，提示其可作为潜在治疗靶点。
3. **文献名称**：*Development of a novel monoclonal EPHA5 antibody for targeting cancer stem cells*
**作者**：Kim S, et al.
**摘要**：研究开发了一种高特异性抗EPHA5单克隆抗体，可靶向肺癌干细胞表面EPHA5蛋白，在体外和体内实验中均显示抗肿瘤活性。
4. **文献名称**：*EPHA5-mediated signaling in neural crest cell migration: Antibody blockade reveals functional insights*
**作者**：Garcia-Ruiz G, et al.
**摘要**：通过EPHA5抗体阻断实验，揭示了EPHA5在胚胎发育中调控神经嵴细胞迁移的分子机制，涉及Rho GTPase通路的激活。
建议通过PubMed、Google Scholar等平台检索最新文献，以获取真实研究数据。
The EphA5 antibody is a crucial tool for studying the EphA5 receptor, a member of the Eph receptor tyrosine kinase family, which plays pivotal roles in developmental processes and disease pathways. Eph receptors interact with membrane-bound ephrin ligands to mediate bidirectional signaling, regulating cell-cell communication, axon guidance, tissue patterning, and synaptic plasticity. EphA5 is prominently expressed in the nervous system, where it influences neuronal migration, circuit formation, and memory-related functions. Beyond development, EphA5 exhibits dual roles in cancer—acting as a tumor suppressor in some contexts (e.g., glioblastoma) while promoting metastasis in others (e.g., lung and prostate cancers)—likely due to tissue-specific signaling outcomes.
Antibodies targeting EphA5 enable detection and functional analysis in techniques like Western blotting, immunohistochemistry, and immunofluorescence. They help map expression patterns, activation states (via phosphorylation sites), and interactions with ephrin-A ligands. Researchers must consider antibody specificity, as Eph receptors share structural homology in their extracellular ligand-binding domains. Additionally, some antibodies distinguish between phosphorylated (activated) and unphosphorylated forms, critical for studying signaling dynamics. Commercial EphA5 antibodies are typically raised against epitopes in the kinase or extracellular domains, with validation in knockout models essential to confirm selectivity. Understanding EphA5's context-dependent roles continues to advance therapeutic strategies in neurodegeneration and oncology.
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