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Rabbit Polyclonal HUWE1 Antibody

  • 中文名: HUWE1抗体
  • 别    名: MULE; Ib772; LASU1; UREB1; HECTH9; URE-B1; ARF-BP1; HSPC272
货号: IPDX12815
Price: ¥1180
数量:
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/10-1/50 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 1/5000-1/10000 Human,Mouse,Rat

产品详情

AliasesMULE; Ib772; LASU1; UREB1; HECTH9; URE-B1; ARF-BP1; HSPC272
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse, Rat
ImmunogenSynthetic peptide of human HUWE1
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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参考文献

以下是关于HUWE1抗体的3篇参考文献及其摘要概括:

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1. **文献名称**: *HUWE1 is a critical regulator of tumor suppression via modulation of MYC degradation*

**作者**: Zhao et al.

**摘要**: 该研究揭示了HUWE1作为E3泛素连接酶通过调控MYC蛋白的泛素化降解在肿瘤抑制中的关键作用。作者利用HUWE1抗体进行免疫共沉淀(Co-IP)和Western blot实验,证实HUWE1与MYC的相互作用及其对细胞增殖的抑制作用。

2. **文献名称**: *HUWE1 interacts with p53 to promote its ubiquitination and degradation in response to DNA damage*

**作者**: Chen et al.

**摘要**: 本研究报道了HUWE1在DNA损伤应答中通过介导p53的泛素化降解调控细胞周期进程。通过HUWE1抗体的染色质免疫沉淀(ChIP)分析,发现HUWE1直接结合p53启动子区域,影响其转录活性。

3. **文献名称**: *HUWE1 regulates neurodevelopmental processes through ubiquitination of substrate proteins*

**作者**: Sánchez-Tena et al.

**摘要**: 该研究利用HUWE1特异性抗体在小鼠脑组织中进行免疫荧光定位,发现HUWE1在神经元分化中通过靶向降解关键蛋白(如N-MYC)调控突触可塑性和神经发育,为神经退行性疾病提供了潜在机制。

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以上文献均涉及HUWE1抗体的实验应用(如Western blot、免疫共沉淀等),并探讨其在癌症、DNA损伤应答或神经发育中的功能。如需具体实验细节或更多文献,建议通过PubMed或Google Scholar以“HUWE1 antibody”为关键词进一步检索。

背景信息

The HUWE1 antibody is a crucial tool for studying the HUWE1 protein, an E3 ubiquitin ligase central to regulating cellular processes like DNA repair, apoptosis, and cell cycle control. HUWE1 (HECT, UBA, and WWE domain-containing E3 ubiquitin protein ligase 1) mediates substrate ubiquitination, targeting proteins such as p53. Myc, and Mcl-1 for proteasomal degradation, thereby influencing tumor suppression, oncogenesis, and cellular homeostasis. Dysregulation of HUWE1 is linked to cancers, neurodevelopmental disorders (e.g., intellectual disability syndromes), and neurodegenerative diseases. Researchers use HUWE1 antibodies in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to analyze its expression, localization, and interactions across tissues. These antibodies help uncover HUWE1’s dual roles—acting as both an oncogene and tumor suppressor depending on context—and its involvement in stress responses. Recent studies also explore its impact on mitochondrial function and synaptic plasticity. By enabling precise detection of HUWE1 isoforms and post-translational modifications, these antibodies are vital for dissecting molecular mechanisms in disease models and evaluating therapeutic strategies targeting ubiquitination pathways.

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