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产品描述：猪碱性成纤维细胞生长因子(bFGF)酶联免疫试剂盒 ，定量检测血清、血浆、组织匀浆样本中的FGF2含量。FGF2即成纤维细胞生长因子2，是重要的促血管生成因子。它参与多种生理过程如胚胎发育、组织修复等。研究机制上，FGF2能与细胞膜上受体结合，激活下游信号通路，调控细胞的增殖、分化、迁移等，在肿瘤血管生成等疾病中作用受关注。试剂盒检测范围为4.7 pg/mL-300 pg/mL，本试剂盒适用于体外研究，如探究猪模型中创伤愈合机制、干细胞培养条件优化或疾病相关生长因子通路分析，为心血管疾病、再生医学及肿瘤生物学等基础研究提供可靠的实验工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：FGF2Fibroblast growth factor 2 ELISA Kit; FGF-2 ELISA Kit; Basic fibroblast growth factor ELISA Kit; bFGF ELISA Kit; Heparin-binding growth factor 2 ELISA Kit; HBGF-2 ELISA Kit; Prostatic growth factor ELISA Kit; Fragments ELISA Kit
缩写：FGF2
Uniprot No.：Q60487
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：4.7 pg/mL-300 pg/mL
灵敏度：1.2 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig bFGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 80-100 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 90-110 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 82-105 | |||||
回收率：
| The recovery of pig bFGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 85-104 | ||||
| EDTA plasma (n=4) | 98 | 92-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 3002.1752.1572.1661.966 1501.5821.6121.5971.397 751.2021.1341.1680.968 37.50.6280.6030.6160.416 18.750.3770.3620.3700.170 9.40.2960.3010.2990.099 4.70.2650.2560.2610.061 00.1960.2040.200 | ||||||
货期：3-5 working days
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