首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠角化细胞生长因子(KGF/FGF-7)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的FGF7含量。FGF7，即成纤维细胞生长因子7，是一种上皮细胞特异性生长因子，主要参与组织发育、修复和维持。其研究机制涉及与FGFR2b受体结合，调节细胞增殖、分化和存活，并在多种生理过程中发挥关键作用。试剂盒检测范围为1.56 pg/mL-100 pg/mL，该产品可广泛应用于上皮细胞生物学、组织再生机制、伤口愈合模型等基础研究领域，也可用于评估药物干预或基因修饰对KGF表达水平的影响。对于细胞实验，可直接检测培养上清中分泌型KGF的浓度；针对动物模型，支持从血清、血浆或特定组织匀浆中定量分析内源性KGF的表达动态，为皮肤、肺、肠道等上皮组织相关研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Fgf7 ELISA Kit; Fgf-7 ELISA Kit; Kgf ELISA Kit; Fibroblast growth factor 7 ELISA Kit; FGF-7 ELISA Kit; Heparin-binding growth factor 7 ELISA Kit; HBGF-7 ELISA Kit; Keratinocyte growth factor ELISA Kit; KGF ELISA Kit
缩写：FGF7
Uniprot No.：P36363
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：1.56 pg/mL-100 pg/mL
灵敏度：0.39 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse KGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 94 | ||||
| Range % | 89-98 | |||||
| 1:200 | Average % | 96 | ||||
| Range % | 91-102 | |||||
| 1:400 | Average % | 90 | ||||
| Range % | 85-96 | |||||
| 1:800 | Average % | 94 | ||||
| Range % | 85-105 | |||||
回收率：
| The recovery of mouse KGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 85-96 | ||||
| EDTA plasma (n=4) | 100 | 95-103 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected1002.5132.5692.5412.349502.1632.1682.1661.974251.6331.6581.6461.45412.50.9920.9990.9960.8046.250.5620.5710.5670.3753.120.3680.3830.3760.1841.560.2720.2910.2820.09000.1900.1930.192 | ||||||
货期：3-5 working days
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