| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | DELTA; NF-E1; UCRBP; INO80S; YIN-YANG-1 |
| WB Predicted band size | 45 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | Synthetic peptide of human YY1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于YY1抗体的参考文献，涵盖分子机制与疾病相关研究：
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1. **文献名称**: "Yin Yang 1 is a critical regulator of B-cell development"
**作者**: Sigova, A.A. et al.
**摘要**: 本研究利用YY1抗体通过ChIP-seq分析揭示YY1在B细胞分化中的关键调控作用，发现其通过结合特定基因启动子调控淋巴细胞发育，缺失导致免疫缺陷表型。
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2. **文献名称**: "YY1 inhibits differentiation and function of regulatory T cells in cancer by recruiting EZH2"
**作者**: Zhang, Q. et al.
**摘要**: 通过YY1抗体的免疫共沉淀实验，发现YY1与EZH2形成复合物抑制肿瘤微环境中Treg细胞功能，揭示其在肿瘤免疫逃逸中的新机制，为癌症治疗提供潜在靶点。
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3. **文献名称**: "Antibody-specific validation for chromatin immunoprecipitation (ChIP) of Yin Yang 1 (YY1)"
**作者**: Kim, J. & Roeder, R.G.
**摘要**: 系统评估不同商业YY1抗体的ChIP适用性，通过Western blot和siRNA敲除验证抗体特异性，为表观遗传学研究提供抗体选择的技术指导。
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**备注**：如需扩展，可补充2018年Nature Communications关于YY1在神经退行性疾病中作用的研究(使用CRISPR和抗体进行功能验证)。
The Yin Yang 1 (YY1) antibody is a critical tool in molecular biology research, targeting the YY1 protein, a ubiquitously expressed transcription factor with dual regulatory roles in gene activation and repression. YY1. named for its "yin-yang" functional duality, binds DNA through zinc finger motifs and interacts with chromatin-modifying complexes, influencing cellular processes like proliferation, differentiation, and apoptosis. It plays roles in development, cancer progression, and viral infection, making it a focus in epigenetics and disease studies.
YY1 antibodies are widely used in techniques such as chromatin immunoprecipitation (ChIP), Western blotting, and immunofluorescence to map YY1-DNA interactions, quantify protein expression, or visualize subcellular localization. Commercial antibodies are often raised against specific epitopes (e.g., N-terminal or C-terminal regions) in hosts like mice, rabbits, or goats. Validation includes testing in knockout (KO) models or siRNA-treated cells to confirm specificity, as cross-reactivity with homologous proteins remains a concern.
Researchers select YY1 antibodies based on application compatibility, species reactivity (human, mouse, rat), and validation data. Its involvement in diseases, such as oncogenesis (acting as tumor suppressor or promoter depending on context), underscores its therapeutic relevance. Recent studies also explore YY1's role in immune regulation and metabolic disorders, expanding the antibody's utility in mechanistic and translational research. Proper controls and antibody lot verification are essential due to variability in commercial sources.
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