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产品描述：小鼠卵泡抑素相关蛋白1(FSTL1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FSTL1含量。FSTL1即卵泡抑素样蛋白 1，是分泌型糖蛋白，属于 FST 家族。它参与胚胎发育、组织修复等过程。研究发现其在肿瘤、心血管疾病等发病机制中发挥作用，可通过多种信号通路影响细胞增殖、凋亡、血管生成等。试剂盒检测范围为0.18 ng/mL-12 ng/mL，适用于探究小鼠模型中FSTL1在生殖生理、炎症相关疾病或组织再生等研究领域的表达水平变化，为解析其分子调控机制提供可靠工具；可满足科研场景下对血清、血浆或组织样本中FSTL1的定量分析需求，如基础生物学研究、疾病模型评估或药物干预效果验证等本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Fstl1 ELISA Kit; Frp ELISA Kit; Fstl ELISA Kit; Tsc36Follistatin-related protein 1 ELISA Kit; Follistatin-like protein 1 ELISA Kit; TGF-beta-inducible protein TSC-36 ELISA Kit
缩写：FSTL1
Uniprot No.：Q62356
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.18 ng/mL-12 ng/mL
灵敏度：0.04 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse FSTL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 88-95 | |||||
| 1:2 | Average % | 90 | ||||
| Range % | 87-94 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 92-97 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 92-99 | |||||
回收率：
| The recovery of mouse FSTL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 98 | 95-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected122.7652.7552.7602.65961.8041.7761.7901.68931.2041.1181.1611.0601.50.6320.6220.6270.5260.750.3540.3670.3610.2600.3750.2330.2450.2390.1380.180.1640.1730.1690.06800.1000.1020.101 | ||||||
货期：3-5 working days
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