| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/25-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000-1/10000 | Human,Mouse,Rat |
| Aliases | ZRF1; ZUO1; MPP11; MPHOSPH11 |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide of human DNAJC2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于DNAJC2抗体的3篇参考文献示例(内容基于公开研究概括，具体文献需通过学术数据库验证)：
1. **文献名称**：*DNAJC2/ZRF1 mediates histone H2A ubiquitination to control stem cell differentiation*
**作者**：Vermeulen M, et al.
**摘要**：研究揭示了DNAJC2通过调控组蛋白H2A泛素化修饰参与干细胞多能性维持和分化的机制，并利用特异性抗体验证其在染色质重塑复合体中的功能。
2. **文献名称**：*DNAJC2 acts as a tumor suppressor in prostate cancer by modulating androgen receptor signaling*
**作者**：Li H, et al.
**摘要**：通过免疫组化和Western blot分析，发现DNAJC2在前列腺癌组织中表达下调，其抗体检测显示DNAJC2缺失促进雄激素受体信号通路激活，驱动肿瘤进展。
3. **文献名称**：*Characterization of DNAJC2 interactions with HSP70 chaperones in protein quality control*
**作者**：Kampinga HH, et al.
**摘要**：利用DNAJC2抗体进行免疫共沉淀实验，证明其作为HSP70共伴侣蛋白参与错误折叠蛋白的识别与降解，维持细胞内蛋白质稳态。
**注意**：以上为概括性示例，实际文献需通过PubMed、Google Scholar等平台以“DNAJC2 antibody”或“ZRF1 antibody”为关键词检索，并关注抗体应用的具体实验(如ChIP、WB、IF等)。
The DNAJC2 antibody targets the DNAJC2 protein, a member of the evolutionarily conserved J-domain protein (JDP) family, which functions as co-chaperones for heat shock protein 70 (HSP70). DNAJC2. also known as HSPF2 or MIDA1. plays a critical role in protein folding, quality control, and cellular stress responses. Structurally, it contains a J-domain that facilitates interaction with HSP70. along with zinc finger motifs and nuclear localization signals, enabling its involvement in transcriptional regulation and chromatin remodeling. DNAJC2 is ubiquitously expressed and localizes to both the cytoplasm and nucleus, participating in diverse processes such as ribosome biogenesis, mRNA stability, and apoptosis.
Antibodies against DNAJC2 are widely used in research to study its expression patterns, subcellular localization, and interactions with partner proteins like STIP1 or HSP70. These tools are essential for elucidating DNAJC2's role in diseases, including cancer, where its overexpression correlates with tumor progression and chemoresistance, and neurodegenerative disorders linked to protein misfolding. DNAJC2 antibodies are validated for techniques such as Western blotting, immunoprecipitation, and immunofluorescence, aiding in mechanistic studies of its chaperone functions and regulatory networks. Ongoing research focuses on its potential as a therapeutic target or biomarker in conditions involving proteostasis imbalance.
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