| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SHC1 |
| Uniprot No | P29353 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-474aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHFNTRTQVTREAISLVCEAVPGAKGATRRRK PCSRPLSSILGRSNLKFAGMPITLTVSTSSLNLMAADCKQIIANHHMQSI SFASGGDPDTMNKLSGGGGRRTRVEGGQLGGEEWTRHGSFVNKPTRGWLH PNDKVMGPGVSYLVRYMGCVEVLQSMRALDAEYVAYVAKDPVNQRACHIL ECPEGLAQDVISTIGQAFELRFKQYLRNPPKLVTPHDRMAGFDGSAWDEE EEEPPDHQYYNDFPGKEPPLGGVVDMRLREGAAPGAARPTAPNAQTPSHL GATLPVGQPVGGDPEVRKQMPPPPPCPAGRELFDDPSYVNVQNLDKARQA VGGAGPPNPAINGSAPRDLFDMKPFEDALRVPPPPQSVSMAEQLRGEPWF HGKLSRREAEALLQLNGDFLVRESTTTPGQYVLTGLQSGQPKHLLLVDPE GVVRTKDHRFESVSHLISYHMDNHLPIISAGSELCLQQPVERKL |
| 预测分子量 | 54 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"A novel transforming protein SHC containing a Src homology 2 domain binds to tyrosine-phosphorylated receptors"**
*作者：Pelicci G, et al. (1992)*
**摘要**：该研究首次克隆了SHC1 cDNA，并通过重组蛋白表达验证了其SH2结构域与激活的酪氨酸磷酸化受体(如EGFR)的结合能力，揭示了SHC1在受体酪氨酸激酶信号转导中的作用。
2. **"Differential tyrosine phosphorylation of Shc proteins by the insulin and epidermal growth factor receptors"**
*作者：Luzi L, et al. (2000)*
**摘要**：通过在大肠杆菌中表达重组SHC1不同亚型(p46/p52/p66)，研究其被胰岛素受体和EGFR磷酸化的差异，阐明了亚型特异性磷酸化对下游信号的影响。
3. **"Shc mediates nerve growth factor-induced differentiation of PC12 cells via the phosphatidylinositol 3-kinase pathway"**
*作者：Okada S, et al. (1997)*
**摘要**：利用重组SHC1蛋白进行体外结合实验，证明其通过TrkA受体介导神经生长因子信号，激活PI3K通路并调控PC12细胞分化。
4. **"Recruitment of Shc by the ITIM-like motif of FcγRIIB reveals divergent signaling pathways from B cell receptor"**
*作者：Ravichandran KS, et al. (1999)*
**摘要**：通过重组SHC1蛋白的磷酸化实验，揭示了其在FcγRIIB受体信号中的调控机制，并解析了磷酸化后与Grb2等适配体蛋白形成复合物的结构基础。
**Background of SHC1 Recombinant Protein**
SHC1 (Src homology 2 domain-containing transforming protein 1) is a critical adaptor protein involved in intracellular signaling pathways, particularly those mediated by receptor tyrosine kinases (RTKs). It belongs to the SHC family, which includes multiple isoforms (p66. p52. and p46) generated through alternative splicing or translation initiation. SHC1 contains a phosphotyrosine-binding (PTB) domain, a central collagen-homology (CH1) region, and a C-terminal Src homology 2 (SH2) domain, enabling interactions with phosphorylated tyrosine residues on activated receptors and downstream signaling molecules.
Functionally, SHC1 bridges activated RTKs (e.g., EGFR, IGF-1R) to downstream effectors like Grb2-SOS complexes, facilitating Ras/MAPK pathway activation. This cascade regulates cell proliferation, differentiation, and survival. The p66 isoform uniquely participates in oxidative stress responses and apoptosis, highlighting SHC1's role in cellular homeostasis and stress adaptation.
Recombinant SHC1 protein is engineered for in vitro and in vivo studies to dissect its molecular interactions, post-translational modifications (e.g., tyrosine phosphorylation), and roles in diseases. Produced via bacterial or mammalian expression systems, it retains functional domains and is often tagged for purification or detection. Its applications include elucidating signaling mechanisms in cancer (where SHC1 is frequently dysregulated), neurodegenerative disorders, and metabolic syndromes. Additionally, it serves as a tool for drug screening, aiming to modulate SHC1-mediated pathways for therapeutic benefit.
In summary, SHC1 recombinant protein is a vital resource for decoding RTK signaling complexity and exploring its implications in health and disease.
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