| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SMNDC1 |
| Uniprot No | O75940 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-238aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMSEDLAKQLASYKAQLQQVEAALSGNGENE DLLKLKKDLQEVIELTKDLLSTQPSETLASSDSFASTQPTHSWKVGDKCM AVWSEDGQCYEAEIEEIDEENGTAAITFAGYGNAEVTPLLNLKPVEEGRK AKEDSGNKPMSKKEMIAQQREYKKKKALKKAQRIKELEQEREDQKVKWQQ FNNRAYSKNKKGQVKRSIFASPESVTGKVGVGTCGIADKPMTQYQDTSKY NVRHLMPQ |
| 预测分子量 | 29 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SMNDC1重组蛋白的3篇代表性文献摘要(示例基于虚构文献，实际文献需通过数据库检索确认)：
---
1. **文献名称**：*Structural and functional analysis of SMNDC1 in spliceosome assembly*
**作者**：Zhang L, et al.
**摘要**：本研究通过大肠杆菌系统表达并纯化了His标签的SMNDC1重组蛋白，结合体外剪接体组装实验，发现SMNDC1通过与U1 snRNP的相互作用促进早期剪接体的形成，揭示了其在RNA剪接中的关键支架功能。
---
2. **文献名称**：*SMNDC1 interacts with Survival Motor Neuron (SMN) protein in vitro*
**作者**：Singh R, et al.
**摘要**：利用GST pull-down和Co-IP实验，验证了重组SMNDC1蛋白与SMN复合体的直接结合，表明其在脊髓性肌萎缩症(SMA)病理中可能通过调控SMN的稳定性参与疾病进程。
---
3. **文献名称**：*High-yield purification and biochemical characterization of recombinant SMNDC1*
**作者**：Wang Y, et al.
**摘要**：优化了昆虫细胞系统中SMNDC1重组蛋白的表达条件，通过离子交换层析获得高纯度蛋白，并证明其具有RNA结合活性，为后续结构生物学研究(如X射线晶体学)奠定了基础。
---
如需具体文献，建议通过PubMed或Google Scholar以关键词“SMNDC1 recombinant protein”或“SMNDC1 purification/function”检索近年研究。
**Background of SMNDC1 Recombinant Protein**
SMNDC1 (Survival Motor Neuron domain-containing protein 1) is a nuclear protein encoded by the *SMNDC1* gene, which belongs to the survival motor neuron (SMN) protein family. While SMN proteins are best known for their role in spliceosome assembly and RNA metabolism, SMNDC1 shares structural homology with SMN but exhibits distinct functional properties. It contains a conserved Tudor domain, a motif implicated in binding methylated arginine residues, and is involved in RNA processing, transcriptional regulation, and cellular localization of mRNA-protein complexes.
The recombinant SMNDC1 protein is engineered using expression systems like *E. coli* or mammalian cells, enabling large-scale production for functional studies. Its recombinant form retains key domains necessary for interactions with spliceosomal components, RNA molecules, and other nuclear proteins. Researchers utilize SMNDC1 recombinant protein to dissect its role in pre-mRNA splicing, particularly its association with small nuclear ribonucleoproteins (snRNPs) and its influence on gene expression networks.
SMNDC1 has also been linked to disease contexts. While mutations in the related *SMN1* gene cause spinal muscular atrophy (SMA), SMNDC1 dysregulation is implicated in cancer progression and neurological disorders, possibly due to aberrant RNA splicing or disrupted nuclear transport. Recombinant SMNDC1 serves as a critical tool for structural studies, inhibitor screening, and elucidating its interplay with epigenetic modifiers. Understanding its molecular mechanisms may reveal therapeutic targets for diseases linked to RNA processing defects.
×
