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产品描述：猪髓过氧化物酶(MPO)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MPO含量。MPO即髓过氧化物酶，是一种存在于中性粒细胞和单核细胞中的血红素蛋白。它在炎症反应和免疫防御中起重要作用，可参与杀灭病原体。其研究机制聚焦于其在氧化应激、炎症损伤等病理过程中的作用，有望为相关疾病治疗提供新方向。试剂盒检测范围为15.6 ng/mL-1000 ng/mL，适用于科研领域中对感染性疾病模型、免疫调控机制、心血管或神经退行性疾病的病理研究，也可用于评估抗炎药物或抗氧化剂对MPO活性的干预效果。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：MPO
Uniprot No.：K7GRV6
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 ng/mL-1000 ng/mL
灵敏度：3.9 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig MPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 91 | ||||
| Range % | 87-99 | |||||
| 1:10 | Average % | 97 | ||||
| Range % | 93-105 | |||||
| 1:20 | Average % | 85 | ||||
| Range % | 82-89 | |||||
| 1:40 | Average % | 90 | ||||
| Range % | 85-98 | |||||
回收率：
| The recovery of pig MPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-95 | ||||
| EDTA plasma (n=4) | 95 | 89-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected10002.2762.1542.2152.0195001.8631.8721.8681.6722501.2971.3071.3021.1061250.7910.8110.8010.60562.50.4520.4690.4610.26531.250.3560.3640.3600.16415.60.2420.2320.2370.04100.1950.1970.196 | ||||||
货期：3-5 working days
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