首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠髓过氧化物酶(MPO)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MPO含量。MPO（髓过氧化物酶）是一种血红素蛋白酶，存在于中性粒细胞中，参与免疫和炎症反应。其研究背景涉及其在多种疾病中的作用，如心血管疾病和肿瘤。研究机制主要关注其基因表达、酶活性及其与宿主防御的相互作用。试剂盒检测范围为0.312 ng/mL-20 ng/mL，该产品广泛应用于炎症机制研究、动脉粥样硬化模型评估、神经退行性疾病研究等科研领域，也可用于药物干预实验中生物标志物的动态监测。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：MPO
Uniprot No.：D3ZGE2
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat MPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-95 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-102 | |||||
回收率：
| The recovery of rat MPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 92 | 87-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected202.3472.2322.2902.109101.4161.3861.4011.22050.9020.8870.8950.7142.50.6020.6120.6070.4261.250.4210.4390.4300.2490.6250.3120.3260.3190.1380.3120.2740.2560.2650.08400.1820.1800.181 | ||||||
货期：3-5 working days
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