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产品描述：小鼠巨噬细胞甘露糖受体1(MRC1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的MRC1含量。MRC1是巨噬细胞上的甘露糖受体，参与先天免疫，识别并结合病原体表面的甘露糖结构，介导巨噬细胞对病原体的吞噬作用。研究显示，MRC1在肿瘤微环境中发挥重要作用，其表达与肿瘤进展和转移密切相关。试剂盒检测范围为0.625 ng/mL-40 ng/mL，特别适用于评估巨噬细胞极化状态、肿瘤免疫微环境分析以及自身免疫性疾病模型中受体表达动态变化的研究本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Mrc1 ELISA Kit; Macrophage mannose receptor 1 ELISA Kit; MMR ELISA Kit; CD antigen CD206 ELISA Kit
缩写：MRC1
Uniprot No.：Q61830
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse MRC1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 81-92 | |||||
| 1:2 | Average % | 100 | ||||
| Range % | 93-104 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-95 | |||||
| 1:8 | Average % | 99 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of mouse MRC1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 85-96 | ||||
| EDTA plasma (n=4) | 100 | 90-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected402.7162.5782.6472.462202.1672.1872.1771.992101.4511.4781.4651.28050.8570.8230.8400.6552.50.5360.5450.5410.3561.250.3710.3590.3650.1800.6250.2740.2660.2700.08500.1880.1820.185 | ||||||
货期：3-5 working days
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